LOCUS       sequence-80730-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible entry vector for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pENTR-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..105
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(369..674)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(471..490)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tggaggcaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
       61 agcaatgctt tcttataatg ccaactttgt acaagaaagc tgggtcggcg cgcccaccct
      121 tgagactaaa gatgagatct aatctgttct ttcatgtttt gttttagaca aaatactata
      181 gtttcttagt ttaaacaaaa gagatagaaa ataagacacg tttataaaag atagcttaaa
      241 tggacagtaa aaagaattga ttctcaagtg attttaggcg ttttgcctcg gcctttttca
      301 ttgtagtaag aagagccaat gtgagaccgt cgaggtgcag actggctgtg tataagggag
      361 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
      421 tggctgagat cagccacttc ttccccgata acggaaaccg gcacactggc catatcggtg
      481 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact
      541 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcggcc gggcgtgtca
      601 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa
      661 accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat
      721 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag
      781 acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga catcatatat
      841 gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca
      901 tacctctttt
//
