LOCUS       sequence-80731-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pFK210B-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..48
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(342..347,348..365,366..372)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(492..511)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gggggacgag cttctagagg atcacaagtt tgtacaaaaa agcaggctcc gcggccgccc
       61 ccttcaccta taggggggaa aaaaaggtag tcatcagata tatattttgg taagaaaata
      121 tagaaatgaa taatttcacg tttaacgaag aggagatgac gtgtgttcct tcgaacccga
      181 gttttgttcg tctataaata gcaccttctc ttctccttct tcctcacttc catcttttta
      241 gcttcactat ctctctataa tcggttttat ctttctctaa gtcacaaccc aaaaaaacaa
      301 agtagagaag aatctgtaag agaccattag gcaccccagg ctttacactt tatgcttccg
      361 gctcgtataa tgtgtggatt ttgagttagg agccgtcgag attttcagga gctaaggaag
      421 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      481 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      541 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      601 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg gcaatgaaag
      661 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
      721 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
      781 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaaggg
//
