LOCUS       sequence-80733-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pFK210B-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(328..352)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
       61 ataaatgtca ggctccctta tacacagcca gtctgcacct cgacggtctc acattggctc
      121 ttcttactac aatgaaaaag gccgaggcaa aacgcctaaa atcacttgag aatcaattct
      181 ttttactgtc catttaagct atcttttata aacgtgtctt attttctatc tcttttgttt
      241 aaactaagaa actatagtat tttgtctaaa acaaaacatg aaagaacaga ttagatctca
      301 tctttagtct caagggtggg cgcgccgacc cagctttctt gtacaaagtg gtgatcctag
      361 ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc agtttcattg
      421 cgcacacacc agaatcctac tgagtttgag tattatggca ttgggaaaac tgtttttctt
      481 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg
      541 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa
      601 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat
      661 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt
      721 aatatgagga gtaaaacact tgtagttgta ccattatgct tattcactag gcaacaaata
      781 tattttcaga cctagaaaag ctgcaaatgt tactgaatac aagtatgtcc tcttgtgt
//
