LOCUS       sequence-80738-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of synthetic 
            trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC123SB-AtTAS1c-B/c
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        326..671
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     614..634
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gagtactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
      301 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
      361 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
      421 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
      481 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      541 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      601 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      661 tggagaggac acgctgaaat cac
//
