LOCUS       sequence-80739-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV plasmid with FEV (Ple67) promoter driving expression of iCre. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1990
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    8..64
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(66..85)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(67..85)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(92..109)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(92..108)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(101..123)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      249..704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(336..355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     546..567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(771..790)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatactcaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
       61 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
      121 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      181 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      241 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      301 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      361 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      421 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      481 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      541 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      601 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
      661 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
      721 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
      781 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
      841 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaaccgtct ccg
//
