LOCUS       sequence-80740-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of synthetic 
            trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC32B-AtTAS1c-B/c
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(152..828)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(195..215)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
       61 tcgcggtgag ttcaggcttt ttcatatctc attgcccccc ggatctgcga aagctcgaga
      121 gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac
      181 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
      241 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
      301 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
      361 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
      421 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
      481 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
      541 tcttggagta gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa
      601 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
      661 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
      721 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
      781 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
      841 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtc
//
