LOCUS       sequence-80741-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of synthetic 
            trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC32B-AtTAS1c-B/c
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        335..679
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     623..643
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
       61 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
      121 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
      181 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
      241 agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata
      301 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
      361 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
      421 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
      481 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
      541 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
      601 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
      661 catttcattt ggagaggacc tcgactctag aggatccccg ggtaccgggc
//
