LOCUS       sequence-80748-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human flag-tagged mTor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pcDNA3-Flag mTOR wt
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             226..504
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             238..510
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
ORIGIN
        1 attgatacgc ccagaccctt ggtgggacgt ctcattcacc agcttctcac agacattggt
       61 cggtaccacc cccaggccct catctaccca ctgacagtgg cttctaagtc taccacgaca
      121 gcccggcaca atgcagccaa caagattctg aagaacatgt gtgagcacag caacaccctg
      181 gtccagcagg ccatgatggt gagcgaggag ctgatccgag tggccatcct ctggcatgag
      241 atgtggcatg aaggcctgga agaggcatct cgtttgtact ttggggaaag gaacgtgaaa
      301 ggcatgtttg aggtgctgga gcccttgcat gctatgatgg aacggggccc ccagactctg
      361 aaggaaacat cctttaatca ggcctatggt cgagatttaa tggaggccca agagtggtgc
      421 aggaagtaca tgaaatcagg gaatgtcaag gacctcaccc aagcctggga cctctattat
      481 catgtgttcc gacgaatctc aaagcagctg cctcagctca catccttaga gctgcaatat
      541 gtttccccaa aacttctgat gtgccgggac cttgaattgg ctgtgccagg aacatatgac
      601 cccaaccagc caatcattcg cattcagtcc atagcaccgt ctttgcaagt catcacatcc
      661 aagcagaggc cccggaaatt gacacttatg ggcagcaacg gacatgagtt tgttttcctt
      721 ctaaaaggcc atgaagatct gcgccaggat gagcgtgtga tgcagctctt cggcctggtt
      781 aacacccttc tggccaatga cccaacatct cttcggaaaa acctcagcat ccagagatac
      841 gctgtcatcc ctttatcgac caactcgggc ctcattggct ggg
//
