LOCUS       sequence-80754-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers 
            WR
  TITLE     Phosphorylation of the PTEN tail acts as an inhibitory switch by 
            preventing its recruitment into a protein complex.
  JOURNAL   J Biol Chem. 2001 Dec 28. 276(52):48627-30.
  PUBMED    11707428
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Dec 28. 276(52):48627-30."
COMMENT     Sequence Label: 809 pcDNA3 GFP PTEN
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(51..70)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(52..70)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(115..318)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(144..164)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        319..698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     870..889
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 actccagtga agagttcttc tcctttactc atggtggcaa gcttgggtct ccctatagtg
       61 agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct
      121 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
      181 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
      241 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
      301 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
      361 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
      421 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
      481 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
      541 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
      601 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
      661 aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac
      721 atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa
      781 gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca
      841 agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
      901 ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat
      961 gtgcgcggaa cccctatttg tttat
//
