LOCUS       sequence-80757-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing the reverse tetracycline 
            transactivator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Hockemeyer D, Soldner F, Cook EG, Gao Q, Mitalipova M, Jaenisch R
  TITLE     A drug-inducible system for direct reprogramming of human somatic 
            cells to pluripotency.
  JOURNAL   Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
  PUBMED    18786421
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Sep 11. 3(3):346-53."
COMMENT     Sequence Label: FUW-M2rtTA
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..90
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     488..508
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(607..717)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 gactcgtctg agggcgaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg
       61 ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag
      121 aatccaggtg gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac
      181 cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc
      241 gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca
      301 ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc
      361 acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa
      421 ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc
      481 gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat
      541 tttgtaatcc agaggttgat tatcgataag cttgatatcg aattgatcct tacttagtta
      601 cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc
      661 aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggc
      721 cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc
      781 gaaaaggaa
//
