LOCUS       sequence-80763-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector for E. coli RtcB RNA ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Peach SE, York K, Hesselberth JR
  TITLE     Global analysis of RNA cleavage by 5'-hydroxyl RNA sequencing.
  JOURNAL   Nucleic Acids Res. 2015 May 22. pii: gkv536.
  PUBMED    26001965
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 22. pii: gkv536."
COMMENT     Sequence Label: pET-53-RtcB
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..166
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    170..194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata ccatggcaca tcaccaccac catcacgtca caagtttgta
      181 caaaaaagca ggctctatga attacgaatt actgaccact gaaaatgccc cggtaaaaat
      241 gtggaccaaa ggcgtgccgg tagaggccga tgcgcgtcag caacttatta atacggcgaa
      301 gatgccgttt attttcaaac atattgcggt aatgcctgat gtacacctgg gtaaaggttc
      361 caccattggt agcgtgatcc cgaccaaagg ggcgattatt ccggcggcgg tgggcgtgga
      421 tattggctgt ggaatgaacg cgctgcgtac cgcgttaacg gcggaagacc tgcctgaaaa
      481 cctggcagag ctgcgtcagg cgattgaaac ggccgtgccg cacgggcgta ccactggccg
      541 ttgtaaacgt gataaaggtg cctgggaaaa tccacctgtt aacgtcgatg ctaaatgggc
      601 tgagcttgaa gccggttatc agtggttaac gcaaaaatat ccccgtttcc tgaataccaa
      661 taactataaa cacctgggaa cgctgggaac cggtaaccac tttattgaaa tctgccttga
      721 tgagtcggac caggtgtgga ttatgctgca ctccggttca cgcggaattg gtaacgccat
      781 cgggacttac tttatcgatc tggcacaaaa agagatgcag gaaacgcttg agacgttgcc
      841 gtcgcgtgat ctggcgtact ttatgga
//
