LOCUS       sequence-80764-e         218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 
            5A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-4CYQ
FEATURES             Location/Qualifiers
     source          1..218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(141..157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(141..157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(154..176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    165..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(189..195,196..213,214..218))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggccatgtct tgaagtgtgt gaaagatcag aatggctgtt acgtggttca gaaatgcatt
       61 gaatgtgtac agccccagtc tttgcaattt atcatcgatg cgtttaaggg acaggagaga
      121 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      181 attccacaca acatacgagc cggaagcata aagtgtaa
//
