LOCUS       sequence-80788-e        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Schirmer EC, Homann OR, Kowal AS, Lindquist S
  TITLE     Dominant gain-of-function mutations in Hsp104p reveal crucial roles 
            for the middle region.
  JOURNAL   Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20.
  PUBMED    14978213
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2004-05"; volume: "15(5)"; pages: "2061-72. Epub 2004 
            Feb 20"
COMMENT     Sequence Label: TM104b(A503V/A509D)
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(68..348)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(369..388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     488..510
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(548..566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctgtgctctg caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa
       61 cagacatact ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc
      121 accaatgccc tccctcttgg ccctctcctt ttcttttttc gaccgaatta attcttaatc
      181 ggcaaaaaaa gaaaagctcc ggatcaagat tgtacgtaag gtgacaagct atttttcaat
      241 aaagaatatc ttccactact gccatctggc gtcataactg caaagtacac atatattacg
      301 atgctgttct attaaatgct tcctatatta tatatatagt aatgtcgtga tctatggtgc
      361 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
      421 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      481 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
      541 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      601 tagacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga tggaataatt
      661 tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt agaaagtaaa
      721 taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt
      781 tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga ttaaatagat
      841 atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt gtggtcttct
      901 acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagag caagataaaa
      961 ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac aaaaact
//
