LOCUS       sequence-80792-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Receiving vector for PUM-HD assembly and expression in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pCMV-TTP(C147R)-GG-PUF
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..72
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
       61 gacgatgaca agatggatct gactgccatc tacgagagcc tcctgtcgct gagccctgac
      121 gtgcccgtgc catccgacca tggagggact gagtccagcc caggctgggg ctcctcggga
      181 ccctggagcc tgagcccctc cgactccagc ccgtctgggg tcacctcccg cctgcctggc
      241 cgctccacca gcctagtgga gggccgcagc tgtggctggg tgcccccacc ccctggcttc
      301 gcaccgctgg ctccccgcct gggccctgag ctgtcaccct cacccacttc gcccactgca
      361 acctccacca ccccctcgcg ctacaagact gagctatgtc ggaccttctc agagagtggg
      421 cgctgccgct acggggccaa gtgccagttt gcccatggcc tgggcgagct gcgccaggcc
      481 aatcgccacc ccaaatacaa gacggaactc cgtcacaagt tctacctcca gggccgctgc
      541 ccctacggct ctcgctgcca cttcatccac aaccctagcg aagacctggc ggccccgggc
      601 caccctcctg tgcttcgcca gagcatcagc ttctccggcc tgccctctgg ccgccggacc
      661 tcaccaccac caccaggcct ggccggccct tccctgtcct ccagctcctt ctcgccctcc
      721 agctccccac caccacctgg ggaccttcca ctgtcaccct ctgccttctc tgctgcccct
      781 ggcacccccc tggctcgaag agatcccacc ccagtctgtt gcccctcctg ccgaagggcc
      841 actcctatca gcgtctgggg gcccttgggt ggcctggttc ggaccccctc tgtacagtcc
      901 ctgggatccg accctgatga atatgccagc agcggcagca gcctgggggg ctctgactct
      961 cccgtcttcg aggcgggagt ttttgcacca ccccagcccg tggcagcccc ccggcgactc
     1021 cccatcttca atcgcatctc tgtttctga
//
