LOCUS       sequence-80793-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T-DNA destination plasmid. Contains the bean yellow dwarf virus 
            cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. 
            Contains attR1 and attR2 sites between the LIR and SIR sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSL
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(712..758)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(747..767)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg cagggagggt
       61 cgtacgaata attcgtatcc aacggaaata cctgatacaa tatacgctcc atcaaatacc
      121 atcacatcgt atatgctttt atagtgtgaa cacctttaac cctagtgggc gggaaatttt
      181 ctactttaaa tctggaccgc tcgtgctaaa gcactcgcga taaggggggg ccacgccggt
      241 aatattaaat tcggcgtggg cccccccttg tcgcaaagac ttcgtcttta agtaaattcc
      301 acgtcatttt ccactatcta ttaaaatgac caaaataccc ctgcctccat gcctccacgc
      361 cggttataag atagagtttg aggcaacccc tcggagtcac aacaacatgc cttctgctag
      421 taagaacttc agactccaat ctaaatatgt tttccttacc tatcccaagt gctcatctca
      481 aagagatgat ttattccagt ttctctggga gaaactcaca ccttttctta ttttcttcct
      541 tggtgttgct tctgagcttc atcaagatgg cactacccac tatcatgctc ttctccagct
      601 tgataaaaaa ccttgtatta gaagacagta ataatgtgtg aagtgaaact tacctctcca
      661 aatgaaatga acttccttat atagaggaag ggggatcctc tagagtcgag gtcctctcca
      721 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
      781 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
      841 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
      901 cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg atggcatttg taggagccac
      961 cttccttttc cactatcttc acaataaagt gacaga
//
