LOCUS       sequence-80795-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based, Golgi-targeted kinase activity reporter for PKD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Kunkel MT, Newton AC
  TITLE     Calcium transduces plasma membrane receptor signals to produce 
            diacylglycerol at Golgi membranes.
  JOURNAL   J Biol Chem. 2010 Jul 23;285(30):22748-52. doi: 
            10.1074/jbc.C110.123133. Epub 2010 Jun 2.
  PUBMED    20519514
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.C110.123133"; journalName: "J Biol Chem"; date: 
            "2010-07-23- 23"; volume: "285"; issue: "30"; pages: "22748-52"
COMMENT     Sequence Label: Golgi-DKAR
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(712..963)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     primer_bind     complement(754..775)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc actccaccag atccaccttc
      181 ggagacagct gcagtcagtt gcctcgacag acctccggat cctcctaact ttttcaccaa
      241 atctttttct tcggctgttt gcttaagtac tactctttgt tcttgtaaca tacctaatcc
      301 ctcgttaaac agacctgtag tatcgttaat ttccacttta aagccaatga caaatttatt
      361 gtttttatcc caaatgatct tgatttcatc tccgtcttgt aaaaggaatt tcgtaccctg
      421 tatcatgcgg ttattattta aatagctcac gttagttccg gtgtggcaat accaaatatc
      481 atctaaacct tgtgccggag attcatacat gcttttgcct acagcatgcc tctttttgaa
      541 aatgaagcaa tgaactcgag acaacctact gtcttcaatt ttacaattgc aatcctcgga
      601 tctaccaatg aaaaatggat tcacaccttg ctgaatctcc aggctttctt gaataatgct
      661 gtcaggcaat ggttttaaag ttaaaaacct accattaccc ttggtaccag ccttgtacag
      721 ctcgtccatg ccgtgagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      781 gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg
      841 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      901 gctgccgtcc tcgatgttgt ggcggatctt gaagtgggcc ttgatgccgt tcttctgctt
      961 gtcggcggtg atatagacgt tgtggctgat gtagttgtac tccagcttgt gtccc
//
