LOCUS       sequence-80796-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based, Golgi-targeted kinase activity reporter for PKD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Kunkel MT, Newton AC
  TITLE     Calcium transduces plasma membrane receptor signals to produce 
            diacylglycerol at Golgi membranes.
  JOURNAL   J Biol Chem. 2010 Jul 23;285(30):22748-52. doi: 
            10.1074/jbc.C110.123133. Epub 2010 Jun 2.
  PUBMED    20519514
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.C110.123133"; journalName: "J Biol Chem"; date: 
            "2010-07-23- 23"; volume: "285"; issue: "30"; pages: "22748-52"
COMMENT     Sequence Label: Golgi-DKAR
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(445..447,448..960)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(487..508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(748..767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggtagatccg aggattgcaa ttgtaaaatt gaagacagta ggttgtctcg agttcattgc
       61 ttcattttca aaaagaggca tgctgtaggc aaaagcatgt atgaatctcc ggcacaaggt
      121 ttagatgata tttggtattg ccacaccgga actaacgtga gctatttaaa taataaccgc
      181 atgatacagg gtacgaaatt ccttttacaa gacggagatg aaatcaagat catttgggat
      241 aaaaacaata aatttgtcat tggctttaaa gtggaaatta acgatactac aggtctgttt
      301 aacgagggat taggtatgtt acaagaacaa agagtagtac ttaagcaaac agccgaagaa
      361 aaagatttgg tgaaaaagtt aggaggatcc ggaggtctgt cgaggcaact gactgcagct
      421 gtctccgaag gtggatctgg tggagtgagc aagggcgagg agctgttcac cggggtggtg
      481 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      541 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      601 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat gtgcttcgcc
      661 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      721 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      781 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      841 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      901 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      961 gacggcagcg tgcagctcgc cgaccact
//
