LOCUS       sequence-80797-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Stage-Zimmermann T, Schmidt U, Silver PA
  TITLE     Factors affecting nuclear export of the 60S ribosomal subunit in 
            vivo.
  JOURNAL   Mol Biol Cell 2000 Nov;11(11):3777-89.
  PUBMED    11071906
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2000-11"; volume: "11(11)"; pages: "3777-89"
COMMENT     Sequence Label: pPS2167
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(26..44)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    86..589
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        626..730
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(949..968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
       61 atgataataa tggtttctta ggacggatcg cttgcctgta acttacacgc gcctcgtatc
      121 ttttaatgat ggaataattt gggaatttac tctgtgttta tttattttta tgttttgtat
      181 ttggatttta gaaagtaaat aaagaaggta gaagagttac ggaatgaaga aaaaaaaata
      241 aacaaaggtt taaaaaattt caacaaaaag cgtactttac atatatattt attagacaag
      301 aaaagcagat taaatagata tacattcgat taacgataag taaaatgtaa aatcacagga
      361 ttttcgtgtg tggtcttcta cacagacaag atgaaacaat tcggcattaa tacctgagag
      421 caggaagagc aagataaaag gtagtatttg ttggcgatcc ccctagagtc ttttacatct
      481 tcggaaaaca aaaactattt tttctttaat ttcttttttt actttctatt tttaatttat
      541 atatttatat taaaaaattt aaattataat tatttttata gcacgtgatg aaaaggaccc
      601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      901 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      961 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actat
//
