LOCUS       sequence-80831-e        6910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Drosophila Ataxin-7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Mohan RD, Dialynas G, Weake VM, Liu J, Martin-Brown S, Florens L, 
            Washburn MP, Workman JL, Abmayr SM
  TITLE     Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B 
            ubiquitination and leads to neural and retinal degeneration.
  JOURNAL   Genes Dev. 2014 Feb 1;28(3):259-72. doi: 10.1101/gad.225151.113.
  PUBMED    24493646
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.225151"; journalName: "Genes Dev"; date: "2014-02-1- 
            1"; volume: "28"; issue: "3"; pages: "259-72"
COMMENT     Sequence Label: pRmHA3-CH2F2-CG9866
FEATURES             Location/Qualifiers
     source          1..6910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..21)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4..20)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..35)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(229..248)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     348..370
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(408..426)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2506..2527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2542..2547,2548..2565,2566..2572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2580..2596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2585..2607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2604..2620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2604..2620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2631..3053
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             6013..6039
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6052..6078
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6091..6114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6118..6141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
       61 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
      181 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      241 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      301 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
      361 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
      421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     2581 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg cgttgcagga
     2641 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
     2701 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
     2761 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata
     2821 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
     2881 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
     2941 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
     3001 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggaattc
     3061 gagctcgcta gcaagcttgg tacccgggga tccatgcccc tgaacgatgt ggcccccgcc
     3121 ggtggtggag cttatccgga aaatggaacc aagcacaagg tgtcgggaat gtggaagccg
     3181 gactcgtcgg ccggctccaa ttccggctcg ggatcggcgg gactatccct gtttagcctg
     3241 cagggccaaa agtggcagca ggtcttcgac atggaaaagg tcatcaagca actgcgaacg
     3301 gctgagaaga cgtatctgcg tggtggaaag aactcctccg ccggcgcaga tcaacaggcg
     3361 aaggtgcagg tccagcgtct gcaatcggcg gacatggcct actacgatct ggcgccgaag
     3421 ctggccagtc gcgatatagt actctgtagt tcctgctcgg gatgttacac gaaggcaggt
     3481 ttccagcacc acattgcgct gcagcatcct agcgtctggg aggccacatc cagcaaagtg
     3541 aatcccaatc caaatgggac agccatatcc agcgactctc gtctcactgc cacggaaatc
     3601 agtcaggagg gcggggctat cgtgccaagt tcgcccacgg atacgctttc ggcctcgacg
     3661 ctctccagtt gctcgaacgg gtccagcagc tcggcttcac tgcagcatcc ggctgctgcg
     3721 ggttcatcca gcaccacgtc gtcttcgtct tcgtcgcgtc acaagagcag cagcagtaaa
     3781 agtagcagtt cctcctcgtc taagggcagc agcagcactt ccgccggaag taggtcgcgt
     3841 tcgaagtcgt caaagaaccg ccaccactcc tcgccagctc cggccctgct ggagtccaat
     3901 cacaaggagt caaagggatc gaagaagagt tcggccatag ccgctcctcc cgtcgtcgct
     3961 gctgttgtta aggaggagcc accatctgtg ggtgctgctc cggctgccat aaccatgttc
     4021 tcctcctcca gcaactcatc ctgcagctta ccgcccactc ccacgttggg agcggcctcc
     4081 agcgaggtgg cgatgggggt caactattca cctgcccagc ttcagacgga tgcggaaaag
     4141 ctgcccgagc tgcagccaaa gaaaaagttg aagggctcca gcgatcatcg aataaaagcg
     4201 gaaaaggaga agaacaaaga taagtctctg gtgaattcct acgagcagca gcaagtgctc
     4261 agcgaactgg accaggcagt gtcttccatc acgggagcgg tttccgccga gcaggtggcg
     4321 cccgatgagg aggaactgcc cttgcccaac acctccgacg agaactccat ttcgcagacg
     4381 ctcgacgaaa tgctggacag caagctgatc aacgagattc tgaataactt cgatgagagc
     4441 gctctggaca catcgcaaca gcagtcacaa cagcaagcgc agaatggatc gctatcgcat
     4501 cccgcacagg ccactaaaag gctgcgcttt gaggacggaa ccattggcga ggattacgcc
     4561 gtgtaccagc agcaggcggt tcagcaggtt tatggcgagg aagagcatca gcaggagctg
     4621 accaccatcg atgccatgca gttgcagcaa ctgatttacc agcagcagca gatgctcgag
     4681 cagcagcaac tgcaagagca gcagcaacag cagcagcagg atcagaagct tcaggatcac
     4741 gagcagcagc gccagttcag cgtctacaac gtgccgtctc tgacggatga ggcaatggtc
     4801 aatccacagc aacagcagct ggaggagcac atgatcctgc ccagtattat atacgagatc
     4861 accgagtcga atcccgtttc catgctggag cagaaggtct tagccgaatt cctaaccaac
     4921 gcgggctacg aaaatgtgga cgtgaatgtc gtgcaatcgg gcggcataca aaccaacttg
     4981 agttctggaa cggaggcaaa ccaactggcg gatgaactgg ccgactttga gttcagtaaa
     5041 ctggagactg ttagcgacac cgtaaagact gttaagctgg aggccaaggc cgccagccag
     5101 ccacccacaa caaatcctca aatcaatcac catccatcgc tggccaatgc caaataccac
     5161 gaggacagct actttaacgt gatgctgtac tccggagcgc ctcgtccctt ggccatgaac
     5221 acgtttggca tggtgaagtt gccccagggc ctgggcgtca ccttccgcaa gaatctgttg
     5281 accacgcgga aggcgaacaa caatttgcta tccctgtcgg gaggtaatca cctgggcgtg
     5341 gtcggtcagc tggcccgcac accgccgccg ccctcgccag cgttaagcaa cggtctgccg
     5401 ggtaagaatc ccgcctcggg cagaaccatt tacgctcagc gatcgaatgt cattgcacaa
     5461 gatcgcctga ggtgcaataa gagggcgctg gtaggaggaa aagctggtgg tggatcaagc
     5521 ggatcgccgg gagtcatgac agccaagcgt ttgaatgaac tgctaatggg aaaggcgaag
     5581 aaggaggaga agccaccacc ggatagcgag gatggcggcg agcagccgac gaaggacaag
     5641 gaacgcgaca aggaggagga caacaagctg accttcagtt tctacgagaa acgccgccga
     5701 ctgttggccg gcaagctgga gcagctgcag cccctcggcc gccccagctt caccctgccg
     5761 ctccagagca accacaacgc cagccaaagt cagagccact cgccacagca gctcctcaaa
     5821 aagcagctgc tgcgcacctt gtccgacagc agcagcaaca tgaacatcag caacatgaac
     5881 aacatcagca atttgaacag cagcaacact cccagcgcca acagcaatcc tccctggaag
     5941 gggaacagtt ccaatccggg aggcaactcc actccaacca gcagcgactt gatgcgcgtc
     6001 ttcgtcggat cctaccccta cgatgttccc gattacgctg gcggccgatc ctacccctac
     6061 gatgttcccg attacgctgg cggccgatcc gactacaagg acgatgatga caagctcgac
     6121 tacaaggacg atgatgacaa gtgactcgac ctgcaggcat gcaagctatt cgatgcacac
     6181 tcacattctt ctcctaatac gataataaaa ctttccatga aaaatatgga aaaatatatg
     6241 aaaattgaga aatccaaaaa actgataaac gctctactta attaaaatag ataaatggga
     6301 gcggcaggaa tggcggagca tggccaagtt cctccgccaa tcagtcgtaa aacagaagtc
     6361 gtggaaagcg gatagaaaga atgttcgatt tgacgggcaa gcatgtctgc tatgtggcgg
     6421 attgcggagg aattgcactg gagaccagca aggttctcat gaccaagaat atagcggtga
     6481 gtgagcggga agctcggttt ctgtccagat cgaactcaaa actagtccag ccagtcgctg
     6541 tcgaaactaa ttaagtaaat gagtttttca tgttagtttc gcgctgagca acaattaagt
     6601 ttatgtttca gttcggctta gatttcgctg aaggacttgc cactttcaat caatacttta
     6661 gaacaaaatc aaaactcatt ctaatagctt ggtgttcatc ttttttttta atgataagca
     6721 ttttgtcgtt tatacttttt atatttcgat attaaaccac ctatgaagtt cattttaatc
     6781 gccagataag caatatattg tgtaaatatt tgtattcttt atcaggaaat tcagggagac
     6841 ggggaagtta ctatctacta aaagccaaac aatttcttac agttttactc tctctactct
     6901 agagtagctt
//
