LOCUS       sequence-80832-e        6159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Drosophila Sgf11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Mohan RD, Dialynas G, Weake VM, Liu J, Martin-Brown S, Florens L, 
            Washburn MP, Workman JL, Abmayr SM
  TITLE     Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B 
            ubiquitination and leads to neural and retinal degeneration.
  JOURNAL   Genes Dev. 2014 Feb 1;28(3):259-72. doi: 10.1101/gad.225151.113.
  PUBMED    24493646
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.225151"; journalName: "Genes Dev"; date: "2014-02-1- 
            1"; volume: "28"; issue: "3"; pages: "259-72"
COMMENT     Sequence Label: pBacPak-FLAG-HIS-Sgf11
FEATURES             Location/Qualifiers
     source          1..6159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             327..451
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(489..1094)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        1158..1249
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1174..1191
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1264..1281
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1291..1314
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1831..1842
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_recomb     1958..3315
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1974..3315)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      3462..3917
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3549..3568)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3759..3780
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3934..3953)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4053..4075
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4113..4131)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4199..4303
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4304..4372,4373..5164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4522..4541)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5335..5923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5824..5843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6077..6094
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccatg
     1261 ggtcatcatc atcaccatca cgattacgga gactacaagg acgatgatga caaggagaac
     1321 ccctacttcc agggcctcga gatgtctgca gccaacatgc cgacgacaac aggagctcag
     1381 ggatcgggaa accaagttcc cacaactagc actacgattg taaaccactt ccgggagttg
     1441 atcaaggaac cgaagaacct cgacgaggcg gccaactatc tgtaccagtc gctgctcgac
     1501 gacgccgtag tcggaatctt taacgagaca catcatctgc gaaagtcagg gaatctggcc
     1561 gccctggacg gagtgccgga ggactcaacc tatcgaatgt gcgagatgcc caacctggac
     1621 atattcggca tctcaacggc caaaaagcca atggactgca cctgccccaa ctgtgatcgc
     1681 ctggtggccg ccgcccgctt cgccccgcac ctggaaaagt gcatgggcat gggtcgtatc
     1741 tcgtcgcgaa ttgcctctcg tcgcttggcc accaaggagg gcgccacatc cgcccacctg
     1801 cactcttcgg gaaataccgg aggcactgat gatgaggatg acgtggactg gtcgtccgac
     1861 aagcgtcgaa agaaatcaaa ccagaactca aggaacaacg gctccaagaa gaacaatggc
     1921 aaaacctttt aggcggccgc ttaattaatt gatccgggtt attagtacat ttattaagcg
     1981 ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa taattcatta
     2041 aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc agcgtcttta
     2101 tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg attagtttca
     2161 aacaagggtt gtttttccga accgatggct ggactatcta atggattttc gctcaacgcc
     2221 acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt ttgtgttttg
     2281 ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt tctttcatca
     2341 ctgtcgttag tgtacaattg actcgacgta aacacgttaa ataaagcttg gacatattta
     2401 acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta
     2461 gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat tgtgtcggct
     2521 aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttatttctga ttgcgggcgt
     2581 ttttgggcgg gtttcaatct aactgtgccc gattttaatt cagacaacac gttagaaagc
     2641 gatggtgcag gcggtggtaa catttcagac ggcaaatcta ctaatggcgg cggtggtgga
     2701 gctgatgata aatctaccat cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc
     2761 ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa atgtctcttt aggcaacaca
     2821 gtcggcacct caactattgt actggtttcg ggcgccgttt ttggtttgac cggtctgaga
     2881 cgagtgcgat ttttttcgtt tctaatagct tccaacaatt gttgtctgtc gtctaaaggt
     2941 gcagcgggtt gaggttccgt cggcattggt ggagcgggcg gcaattcaga catcgatggt
     3001 ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc
     3061 gccggtataa tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc
     3121 gccgctggct gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat
     3181 tcaatattat aattggaata caaatcgtaa aaatctgcta taagcattgt aatttcgcta
     3241 tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag caattgtatt gtaaagagat
     3301 tgtctcaagc tcggatcgat cccgcacgcc gataacaagc cttttcattt ttactacagc
     3361 attgtagtgg cgagacactt cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc
     3421 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     3481 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3541 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3601 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3661 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3721 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3781 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     3841 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3901 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3961 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4021 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4081 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4141 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4201 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4261 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4321 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4381 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4441 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4501 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4561 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4621 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4681 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4741 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4801 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4861 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4921 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4981 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5041 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5101 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5161 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5221 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5341 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5401 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5461 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     5521 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5581 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5641 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5701 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5761 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5821 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5881 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5941 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6001 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6061 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6121 accgcctctc cccgcgcgtt ggccgattca ttaatgcag
//
