LOCUS       sequence-80833-e        5860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Drosophila E(y)2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Mohan RD, Dialynas G, Weake VM, Liu J, Martin-Brown S, Florens L, 
            Washburn MP, Workman JL, Abmayr SM
  TITLE     Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B 
            ubiquitination and leads to neural and retinal degeneration.
  JOURNAL   Genes Dev. 2014 Feb 1;28(3):259-72. doi: 10.1101/gad.225151.113.
  PUBMED    24493646
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.225151"; journalName: "Genes Dev"; date: "2014-02-1- 
            1"; volume: "28"; issue: "3"; pages: "259-72"
COMMENT     Sequence Label: pBacPak-HA-E(y)2
FEATURES             Location/Qualifiers
     source          1..5860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             327..451
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(489..1094)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        1158..1249
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1174..1191
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1268..1294
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1295..1321
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_recomb     1659..3016
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1675..3016)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      3163..3618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3250..3269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3460..3481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3635..3654)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3754..3776
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3814..3832)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3900..4004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4005..4073,4074..4865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4223..4242)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5036..5624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5525..5544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5778..5795
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggaggctag
     1261 caccatgtac ccatatgatg tccctgatta cgcatatcct tacgacgtac cagactatgc
     1321 tctcgagatg agcacttccg gcgcagttga tcagtacacg gtgctaaccg gcgaccgttc
     1381 caagatcaag gatttgctct gcagccgcct tacggaatgt ggctggcggg acgaggtgcg
     1441 tctgatgtgc cgcaacatcc tgatggagaa gggcaccaac aacagcttca ccgtagagca
     1501 gctgatcgcc gaggtaacac ccaaggcacg caccctggtc ccggatgccg tcaaaaagga
     1561 gctgcttatg aagatacgca ccattctcac ggaaattgag gaggaacccg atgagccaga
     1621 ggacgaatcc taagcggccg cttaattaat tgatccgggt tattagtaca tttattaagc
     1681 gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt
     1741 aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt
     1801 atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc
     1861 aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc
     1921 cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt
     1981 gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc
     2041 actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aataaagctt ggacatattt
     2101 aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt
     2161 agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc
     2221 taacacgtcc gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg
     2281 tttttgggcg ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag
     2341 cgatggtgca ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg
     2401 agctgatgat aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg
     2461 cggaggtggt ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac
     2521 agtcggcacc tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag
     2581 acgagtgcga tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg
     2641 tgcagcgggt tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg
     2701 tggtggtggt ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc
     2761 cgccggtata atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg
     2821 cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa
     2881 ttcaatatta taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct
     2941 atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga
     3001 ttgtctcaag ctcggatcga tcccgcacgc cgataacaag ccttttcatt tttactacag
     3061 cattgtagtg gcgagacact tcgctgtcgt cgcctgatgc ggtattttct ccttacgcat
     3121 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     3181 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3241 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3301 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3361 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3421 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3481 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     3541 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3601 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3661 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3721 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3781 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     3841 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3901 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3961 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4021 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4081 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4141 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4201 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4261 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4321 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4381 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4441 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4501 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4561 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4621 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4681 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4741 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4801 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4861 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4921 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4981 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5041 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5101 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5161 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5221 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5281 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5341 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5401 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5461 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5521 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5581 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5641 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5701 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5761 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5821 aaccgcctct ccccgcgcgt tggccgattc attaatgcag
//
