LOCUS       sequence-80834-e        8871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of human TDG C-terminally fused to GyrA intein,
            SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-hTDG-IntE2
FEATURES             Location/Qualifiers
     source          1..8871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1334..1927
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             2444..3100
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(3149..3167)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3163..3210
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3270..3374
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3375..3443,3444..4235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3593..3612)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4277..4790)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4472..4493)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4684..4703
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4901..5489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5390..5409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5643..5660
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5682..5701)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5842..5864
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5858..6049)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6606..7688)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7650..7669
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7689..7766)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      7919..8005
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8102..8188
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8285..8371
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8468..8554
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8651..8737
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataaccat ggaagcggag aacgcgggca gctattccct
      121 tcagcaagct caagcttttt atacgtttcc atttcaacaa ctgatggctg aagctcctaa
      181 tatggcagtt gtgaatgaac agcaaatgcc agaagaagtt ccagccccag ctcctgctca
      241 ggaaccagtg caagaggctc caaaaggaag aaaaagaaaa cccagaacaa cagaaccaaa
      301 acaaccagtg gaacccaaaa aacctgttga gtcaaaaaaa tctggcaagt ctgcaaaatc
      361 aaaagaaaaa caagaaaaaa ttacagacac atttaaagta aaaagaaaag tagaccgttt
      421 taatggtgtt tcagaagctg aacttctgac caagactctc cccgatattt tgaccttcaa
      481 tctggacatt gtcattattg gcataaaccc gggactaatg gctgcttaca aagggcatca
      541 ttaccctgga cctggaaacc atttttggaa gtgtttgttt atgtcagggc tcagtgaggt
      601 ccagctgaac catatggatg atcacactct accagggaag tatggtattg gatttaccaa
      661 catggtggaa aggaccacgc ccggcagcaa agatctctcc agtaaagaat ttcgtgaagg
      721 aggacgtatt ctagtacaga aattacagaa atatcagcca cgaatagcag tgtttaatgg
      781 aaaatgtatt tatgaaattt ttagtaaaga agtttttgga gtaaaggtta agaacttgga
      841 atttgggctt cagccccata agattccaga cacagaaact ctctgctatg ttatgccatc
      901 atccagtgca agatgtgctc agtttcctcg agcccaagac aaagttcatt actacataaa
      961 actgaaggac ttaagagatc agttgaaagg cattgaacga aatatggacg ttcaagaggt
     1021 gcaatataca tttgacctac agcttgccca agaggatgca aagaagatgg ctgttaagga
     1081 agaaaaatat gatccaggtt atgaggcagc atatggtggt gcttacggag aaaatccatg
     1141 cagcagtgaa ccttgtggct tctcttcaaa tgggctaatt gagagcgtgg agttaagagg
     1201 agaatcagct ttcagtggca ttcctaatgg gcagtggatg acccagtcat ttacagacca
     1261 aattccttcc tttagtaatc actgtggaac acaagaacag gaagaagaaa gccatgctga
     1321 attcctcgag atgtgcatct gcggagatgc actagttgcc ctacccgagg gcgagtcggt
     1381 acgcatcgcc gacatcgtgc cgggtgcgcg gcccaacagt gacaacgcca tcgacctgaa
     1441 agtccttgac cggcatggca atcccgtgct cgccgaccgg ctgttccact ccggcgagca
     1501 tccggtgtac acggtgcgta cggtcgaagg tctgcgtgtg acgggcaccg cgaaccaccc
     1561 gttgttgtgt ttggtcgacg tcgccggggt gccgaccctg ctgtggaagc tgatcgacga
     1621 aatcaagccg ggcgattacg cggtgattca acgcagcgca ttcagcgtcg actgtgcagg
     1681 ttttgcccgc gggaaacccg aatttgcgcc cacaacctac acagtcggcg tccctggact
     1741 ggtgcgtttc ttggaagcac accaccgaga cccggacgcc caagctatcg ccgacgagct
     1801 gaccgacggg cggttctact acgcgaaagt cgccagtgtc accgacgccg gcgtgcagcc
     1861 ggtgtatagc cttcgtgtcg acacggcaga ccacgcgttt atcacgaacg ggttcgtcag
     1921 ccacgctact ggcctcaccg gtctaagctt agcatccatg tcggggatcg ccctcagcag
     1981 actcgcccag gagaggaaag catggaggaa agaccaccca tttggtttcg tggctgtccc
     2041 aacaaaaaat cccgatggca cgatgaacct catgaactgg gagtgcgcca ttccgggaaa
     2101 gaaagggact ccgtgggaag gaggcttgtt taaactacgg atgcttttca aagatgatta
     2161 tccatcttcg ccaccaaaat gtaaattcga accaccatta tttcacccga atgtgtaccc
     2221 ttcggggaca gtgtgcctgt ccatcttaga ggaggacaag gactggaggc cagccatcac
     2281 aatcaaacag atcctattag gaatacagga acttctaaat gaaccaaata tccaagaccc
     2341 agctcaagca gaggcctaca cgatttactg ccaaaacaga gtggagtacg agaaaagggt
     2401 ccgagcacaa gccaagaagt ttgcgccctc atcaggatcc ggaatgtccc ctatactagg
     2461 ttattggaaa attaagggcc ttgtgcaacc cactcgactt cttttggaat atcttgaaga
     2521 aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat aaatggcgaa acaaaaagtt
     2581 tgaattgggt ttggagtttc ccaatcttcc ttattatatt gatggtgatg ttaaattaac
     2641 acagtctatg gccatcatac gttatatagc tgacaagcac aacatgttgg gtggttgtcc
     2701 aaaagagcgt gcagagattt caatgcttga aggagcggtt ttggatatta gatacggtgt
     2761 ttcgagaatt gcatatagta aagactttga aactctcaaa gttgattttc ttagcaagct
     2821 acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat aaaacatatt taaatggtga
     2881 tcatgtaacc catcctgact tcatgttgta tgacgctctt gatgttgttt tatacatgga
     2941 cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt aaaaaacgta ttgaagctat
     3001 cccacaaatt gataagtact tgaaatccag caagtatata gcatggcctt tgcagggctg
     3061 gcaagccacg tttggtggtg gcgaccatcc tccaaaatag atccggctgc taacaaagcc
     3121 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     3181 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggataacta
     3241 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3301 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3361 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3421 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3481 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3541 agagttttcg ccccgaagaa cgttctccaa tgatgagcac ttttaaagtt ctgctatgtg
     3601 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3661 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3721 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3781 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3841 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3901 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3961 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4021 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4081 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4141 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4201 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4261 tactttagat tgatttaccc cggttgataa tcagaaaagc cccaaaaaca ggaagattgt
     4321 ataagcaaat atttaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
     4381 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4441 agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4501 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4561 tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4621 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4681 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4741 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct
     4801 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4861 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4921 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4981 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5041 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5101 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5161 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5221 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5281 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5521 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5641 gcagcgagtc agtgagcgag gaagctatgg tgcactctca gtacaatctg ctctgatgcc
     5701 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     5761 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5821 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5881 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgcagc gattcacaga
     5941 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     6001 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     6061 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     6121 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     6181 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagccgaacg
     6241 ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc
     6301 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga
     6361 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa
     6421 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa
     6481 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     6541 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     6601 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6661 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     6721 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     6781 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     6841 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
     6901 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     6961 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     7021 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     7081 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     7141 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     7201 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     7261 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     7321 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     7381 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     7441 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     7501 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     7561 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     7621 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     7681 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     7741 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     7801 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     7861 tgcatgccgg catgccgccc tttcgtcttc aagaattaat tcccaattcc ccaggcatca
     7921 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     7981 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
     8041 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca
     8101 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     8161 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     8221 acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag
     8281 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     8341 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
     8401 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga attaattccc
     8461 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     8521 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     8581 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggaattaatt
     8641 ccccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     8701 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     8761 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggaattg
     8821 gggatcggaa ttaattcccg gtttaaaccg gggatctcga tcccgcgaaa t
//
