LOCUS       sequence-80841-e        9543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA
            intein, SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-hXRCC1-K176R-IntE2
FEATURES             Location/Qualifiers
     source          1..9543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2006..2599
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             3116..3772
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(3821..3839)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3835..3882
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3942..4046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4047..4115,4116..4907)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4265..4284)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4949..5462)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5144..5165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5356..5375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5573..6161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6062..6081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6315..6332
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6354..6373)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6514..6536
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6530..6721)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7278..8360)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8322..8341
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8361..8438)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      8591..8677
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8774..8860
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8957..9043
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9140..9226
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9323..9409
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataaccat gggaccggag atccgcctcc gccatgtcgt
      121 gtcctgcagc agccaggact cgactcactg tgcagaaaat cttctcaagg cagacactta
      181 ccgaaaatgg cgggcagcca aggcaggcga gaagaccatc tctgtggtcc tacagttgga
      241 gaaggaggag cagatacaca gtgtggacat tgggaatgat ggctcagctt tcgtggaggt
      301 gctggtgggc agttcagctg gaggcgctgg ggagcaagac tatgaggtcc ttctggtcac
      361 ctcatctttc atgtcccctt ccgagagccg cagtggctca aaccccaacc gcgttcgcat
      421 gtttgggcct gacaagctgg tccgggcagc cgccgagaag cgctgggacc gggtcaaaat
      481 tgtttgcagc cagccctaca gcaaggactc cccctttggc ttgagttttg tacggtttca
      541 tagcccccca gacaaagatg aggcagaggc cccgtcccag aaggtgacag tgaccaagct
      601 tggccagttc cgtgtgaggg aggaggatga gagcgccaac tctctgaggc cgggggctct
      661 cttcttcagc cggatcaaca agacatcccc agtcacagcc agcgacccgg caggacctag
      721 ctatgcagct gctaccctcc aggcttctag tgctgcctcc tcagcctctc cagtctccag
      781 ggccataggc agcacctcca agccccagga gtctcccaaa gggaagagga agttggattt
      841 gaaccaagaa gaaaagaaga cccccagcaa accaccagcc cagctgtcgc catctgttcc
      901 caagagacct aaattgccag ctccaactcg taccccagcc acagccccag tccctgcccg
      961 agcacagggg gcagtgacag gcaaaccccg aggagaaggc accgagccca gacgaccccg
     1021 agctggccca gaggagctgg ggaagatcct tcagggtgtg gtagtggtgc tgagtggctt
     1081 ccagaacccc ttccgctccg agctgcgaga taaggcccta gagcttgggg ccaagtatcg
     1141 gccagactgg acccgggaca gcacgcacct catctgtgcc tttgccaaca cccccaagta
     1201 cagccaggtc ctaggcctgg gaggccgcat cgtgcgtaag gagtgggtgc tggactgtca
     1261 ccgcatgcgt cggcggctgc cctcccggag gtacctcatg gcagggccag gttccagcag
     1321 tgaggaggat gaggcctctc acagcggtgg cagcggagat gaagccccca agcttcctca
     1381 gaagcaaccc cagaccaaaa ccaagcccac tcaggcagct ggacccagct caccccagaa
     1441 gcccccaacc cctgaagaga ccaaagcagc ctcaccagtg ctccaggaag atatagacat
     1501 tgagggggta cagtcagaag gacaggacaa tggggcggaa gattctgggg acacagagga
     1561 tgagctgagg agggtggcag agcagaagga acacagactg ccccctggcc aggaggagaa
     1621 tggggaagac ccgtatgcag gctccacgga tgagaacacg gacagtgagg aacaccagga
     1681 gcctcctgat ctgccagtcc ctgagctccc agatttcttc cagggcaagc acttctttct
     1741 ttacggggag ttccctgggg acgagcggcg gaaactcatc cgatacgtca cagccttcaa
     1801 tggggagctc gaggactata tgagtgaccg ggttcagttt gtgatcacag cacaggaatg
     1861 ggatcccagc tttgaggagg ccctgatgga caacccctcc ctggcattcg ttcgtccccg
     1921 atggatctac agttgcaatg agaagcagaa gttacttcct caccagctct atggggtggt
     1981 gccgcaagcc gaattcctcg agatgtgcat ctgcggagat gcactagttg ccctacccga
     2041 gggcgagtcg gtacgcatcg ccgacatcgt gccgggtgcg cggcccaaca gtgacaacgc
     2101 catcgacctg aaagtccttg accggcatgg caatcccgtg ctcgccgacc ggctgttcca
     2161 ctccggcgag catccggtgt acacggtgcg tacggtcgaa ggtctgcgtg tgacgggcac
     2221 cgcgaaccac ccgttgttgt gtttggtcga cgtcgccggg gtgccgaccc tgctgtggaa
     2281 gctgatcgac gaaatcaagc cgggcgatta cgcggtgatt caacgcagcg cattcagcgt
     2341 cgactgtgca ggttttgccc gcgggaaacc cgaatttgcg cccacaacct acacagtcgg
     2401 cgtccctgga ctggtgcgtt tcttggaagc acaccaccga gacccggacg cccaagctat
     2461 cgccgacgag ctgaccgacg ggcggttcta ctacgcgaaa gtcgccagtg tcaccgacgc
     2521 cggcgtgcag ccggtgtata gccttcgtgt cgacacggca gaccacgcgt ttatcacgaa
     2581 cgggttcgtc agccacgcta ctggcctcac cggtctaagc ttagcatcca tgtcggggat
     2641 cgccctcagc agactcgccc aggagaggaa agcatggagg aaagaccacc catttggttt
     2701 cgtggctgtc ccaacaaaaa atcccgatgg cacgatgaac ctcatgaact gggagtgcgc
     2761 cattccggga aagaaaggga ctccgtggga aggaggcttg tttaaactac ggatgctttt
     2821 caaagatgat tatccatctt cgccaccaaa atgtaaattc gaaccaccat tatttcaccc
     2881 gaatgtgtac ccttcgggga cagtgtgcct gtccatctta gaggaggaca aggactggag
     2941 gccagccatc acaatcaaac agatcctatt aggaatacag gaacttctaa atgaaccaaa
     3001 tatccaagac ccagctcaag cagaggccta cacgatttac tgccaaaaca gagtggagta
     3061 cgagaaaagg gtccgagcac aagccaagaa gtttgcgccc tcatcaggat ccggaatgtc
     3121 ccctatacta ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga
     3181 atatcttgaa gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg
     3241 aaacaaaaag tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga
     3301 tgttaaatta acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt
     3361 gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat
     3421 tagatacggt gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt
     3481 tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata
     3541 tttaaatggt gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt
     3601 tttatacatg gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg
     3661 tattgaagct atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc
     3721 tttgcagggc tggcaagcca cgtttggtgg tggcgaccat cctccaaaat agatccggct
     3781 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     3841 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     3901 tccggataac tacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4201 gtaagatcct tgagagtttt cgccccgaag aacgttctcc aatgatgagc acttttaaag
     4261 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     4321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4921 tttactcata tatactttag attgatttac cccggttgat aatcagaaaa gccccaaaaa
     4981 caggaagatt gtataagcaa atatttaaat tgtaaacgtt aatattttgt taaaattcgc
     5041 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     5101 ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     5161 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     5221 tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc
     5281 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     5341 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     5401 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     5461 gtaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5521 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5581 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5641 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5701 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5761 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5821 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5881 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5941 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6001 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6061 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6121 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6181 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6241 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6301 gaacgaccga gcgcagcgag tcagtgagcg aggaagctat ggtgcactct cagtacaatc
     6361 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     6421 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6481 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6541 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca
     6601 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     6661 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     6721 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     6781 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     6841 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     6901 gccagccgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg
     6961 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt
     7021 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt
     7081 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga
     7141 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
     7201 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta
     7261 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7321 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt
     7381 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
     7441 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
     7501 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
     7561 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
     7621 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
     7681 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
     7741 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg
     7801 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
     7861 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
     7921 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
     7981 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
     8041 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg
     8101 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg
     8161 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg
     8221 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg
     8281 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
     8341 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
     8401 gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt
     8461 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     8521 cgcaaggaat ggtgcatgcc ggcatgccgc cctttcgtct tcaagaatta attcccaatt
     8581 ccccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     8641 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     8701 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggaatta
     8761 attccccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     8821 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     8881 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa
     8941 ttaattcccc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     9001 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     9061 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     9121 gaattaattc cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     9181 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     9241 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     9301 caggaattaa ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     9361 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
     9421 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
     9481 tgccaggaat tggggatcgg aattaattcc cggtttaaac cggggatctc gatcccgcga
     9541 aat
//
