LOCUS       sequence-80843-e        8955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of human XRCC1-K176R C-terminally fused to 
            SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-hXRCC1-K176R-PreE2
FEATURES             Location/Qualifiers
     source          1..8955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1997..2020
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2528..3184
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(3233..3251)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3247..3294
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3354..3458
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3459..3527,3528..4319)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3677..3696)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4361..4874)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4556..4577)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4768..4787
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4985..5573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5474..5493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5727..5744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5766..5785)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5926..5948
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5942..6133)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6690..7772)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7734..7753
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7773..7850)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      8003..8089
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8186..8272
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8369..8455
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8552..8638
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8735..8821
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataaccat gggaccggag atccgcctcc gccatgtcgt
      121 gtcctgcagc agccaggact cgactcactg tgcagaaaat cttctcaagg cagacactta
      181 ccgaaaatgg cgggcagcca aggcaggcga gaagaccatc tctgtggtcc tacagttgga
      241 gaaggaggag cagatacaca gtgtggacat tgggaatgat ggctcagctt tcgtggaggt
      301 gctggtgggc agttcagctg gaggcgctgg ggagcaagac tatgaggtcc ttctggtcac
      361 ctcatctttc atgtcccctt ccgagagccg cagtggctca aaccccaacc gcgttcgcat
      421 gtttgggcct gacaagctgg tccgggcagc cgccgagaag cgctgggacc gggtcaaaat
      481 tgtttgcagc cagccctaca gcaaggactc cccctttggc ttgagttttg tacggtttca
      541 tagcccccca gacaaagatg aggcagaggc cccgtcccag aaggtgacag tgaccaagct
      601 tggccagttc cgtgtgaggg aggaggatga gagcgccaac tctctgaggc cgggggctct
      661 cttcttcagc cggatcaaca agacatcccc agtcacagcc agcgacccgg caggacctag
      721 ctatgcagct gctaccctcc aggcttctag tgctgcctcc tcagcctctc cagtctccag
      781 ggccataggc agcacctcca agccccagga gtctcccaaa gggaagagga agttggattt
      841 gaaccaagaa gaaaagaaga cccccagcaa accaccagcc cagctgtcgc catctgttcc
      901 caagagacct aaattgccag ctccaactcg taccccagcc acagccccag tccctgcccg
      961 agcacagggg gcagtgacag gcaaaccccg aggagaaggc accgagccca gacgaccccg
     1021 agctggccca gaggagctgg ggaagatcct tcagggtgtg gtagtggtgc tgagtggctt
     1081 ccagaacccc ttccgctccg agctgcgaga taaggcccta gagcttgggg ccaagtatcg
     1141 gccagactgg acccgggaca gcacgcacct catctgtgcc tttgccaaca cccccaagta
     1201 cagccaggtc ctaggcctgg gaggccgcat cgtgcgtaag gagtgggtgc tggactgtca
     1261 ccgcatgcgt cggcggctgc cctcccggag gtacctcatg gcagggccag gttccagcag
     1321 tgaggaggat gaggcctctc acagcggtgg cagcggagat gaagccccca agcttcctca
     1381 gaagcaaccc cagaccaaaa ccaagcccac tcaggcagct ggacccagct caccccagaa
     1441 gcccccaacc cctgaagaga ccaaagcagc ctcaccagtg ctccaggaag atatagacat
     1501 tgagggggta cagtcagaag gacaggacaa tggggcggaa gattctgggg acacagagga
     1561 tgagctgagg agggtggcag agcagaagga acacagactg ccccctggcc aggaggagaa
     1621 tggggaagac ccgtatgcag gctccacgga tgagaacacg gacagtgagg aacaccagga
     1681 gcctcctgat ctgccagtcc ctgagctccc agatttcttc cagggcaagc acttctttct
     1741 ttacggggag ttccctgggg acgagcggcg gaaactcatc cgatacgtca cagccttcaa
     1801 tggggagctc gaggactata tgagtgaccg ggttcagttt gtgatcacag cacaggaatg
     1861 ggatcccagc tttgaggagg ccctgatgga caacccctcc ctggcattcg ttcgtccccg
     1921 atggatctac agttgcaatg agaagcagaa gttacttcct caccagctct atggggtggt
     1981 gccgcaagcc gaattcctcg aggttctgtt ccagggtcca ggtggcggaa gcttagcatc
     2041 catgtcgggg atcgccctca gcagactcgc ccaggagagg aaagcatgga ggaaagacca
     2101 cccatttggt ttcgtggctg tcccaacaaa aaatcccgat ggcacgatga acctcatgaa
     2161 ctgggagtgc gccattccgg gaaagaaagg gactccgtgg gaaggaggct tgtttaaact
     2221 acggatgctt ttcaaagatg attatccatc ttcgccacca aaatgtaaat tcgaaccacc
     2281 attatttcac ccgaatgtgt acccttcggg gacagtgtgc ctgtccatct tagaggagga
     2341 caaggactgg aggccagcca tcacaatcaa acagatccta ttaggaatac aggaacttct
     2401 aaatgaacca aatatccaag acccagctca agcagaggcc tacacgattt actgccaaaa
     2461 cagagtggag tacgagaaaa gggtccgagc acaagccaag aagtttgcgc cctcatcagg
     2521 atccggaatg tcccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg
     2581 acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg
     2641 tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta
     2701 tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa
     2761 gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc
     2821 ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct
     2881 caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg
     2941 tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc
     3001 tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg
     3061 ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta
     3121 tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa
     3181 atagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     3241 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     3301 ggaggaacta tatccggata actacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3361 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3421 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3481 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3541 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3601 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgttct ccaatgatga
     3661 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     3721 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3781 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3841 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3901 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3961 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4021 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4081 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4141 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4201 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4261 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4321 tgtcagacca agtttactca tatatacttt agattgattt accccggttg ataatcagaa
     4381 aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg ttaatatttt
     4441 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     4501 cggcaaaatc ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt
     4561 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     4621 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag
     4681 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     4741 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     4801 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     4861 gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5101 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5641 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5701 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagct atggtgcact
     5761 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     5821 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5881 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5941 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     6001 cgtggtcgtg cagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     6061 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     6121 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
     6181 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     6241 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
     6301 ctcagggtca atgccagccg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc
     6361 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt
     6421 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc
     6481 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt
     6541 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga
     6601 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
     6661 tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6721 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6781 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
     6841 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
     6901 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc
     6961 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
     7021 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
     7081 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
     7141 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
     7201 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
     7261 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca
     7321 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc
     7381 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
     7441 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
     7501 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc
     7561 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg
     7621 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca
     7681 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac
     7741 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc
     7801 gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg
     7861 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     7921 gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc gccctttcgt cttcaagaat
     7981 taattcccaa ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     8041 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
     8101 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
     8161 tgccaggaat taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     8221 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     8281 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     8341 aactgccagg aattaattcc ccaggcatca aataaaacga aaggctcagt cgaaagactg
     8401 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     8461 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     8521 ataaactgcc aggaattaat tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     8581 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     8641 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     8701 gccataaact gccaggaatt aattccccag gcatcaaata aaacgaaagg ctcagtcgaa
     8761 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     8821 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     8881 cccgccataa actgccagga attggggatc ggaattaatt cccggtttaa accggggatc
     8941 tcgatcccgc gaaat
//
