LOCUS       sequence-80851-e        9066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA
            intein and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pTXG-hXRCC1-K176R
FEATURES             Location/Qualifiers
     source          1..9066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2006..2599
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             2639..3295
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(3344..3362)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3358..3405
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3465..3569
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3570..3638,3639..4430)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3788..3807)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4472..4985)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4667..4688)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4879..4898
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5096..5684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5585..5604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5838..5855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5877..5896)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6037..6059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6053..6244)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6801..7883)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7845..7864
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7884..7961)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      8114..8200
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8297..8383
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8480..8566
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8663..8749
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8846..8932
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataaccat gggaccggag atccgcctcc gccatgtcgt
      121 gtcctgcagc agccaggact cgactcactg tgcagaaaat cttctcaagg cagacactta
      181 ccgaaaatgg cgggcagcca aggcaggcga gaagaccatc tctgtggtcc tacagttgga
      241 gaaggaggag cagatacaca gtgtggacat tgggaatgat ggctcagctt tcgtggaggt
      301 gctggtgggc agttcagctg gaggcgctgg ggagcaagac tatgaggtcc ttctggtcac
      361 ctcatctttc atgtcccctt ccgagagccg cagtggctca aaccccaacc gcgttcgcat
      421 gtttgggcct gacaagctgg tccgggcagc cgccgagaag cgctgggacc gggtcaaaat
      481 tgtttgcagc cagccctaca gcaaggactc cccctttggc ttgagttttg tacggtttca
      541 tagcccccca gacaaagatg aggcagaggc cccgtcccag aaggtgacag tgaccaagct
      601 tggccagttc cgtgtgaggg aggaggatga gagcgccaac tctctgaggc cgggggctct
      661 cttcttcagc cggatcaaca agacatcccc agtcacagcc agcgacccgg caggacctag
      721 ctatgcagct gctaccctcc aggcttctag tgctgcctcc tcagcctctc cagtctccag
      781 ggccataggc agcacctcca agccccagga gtctcccaaa gggaagagga agttggattt
      841 gaaccaagaa gaaaagaaga cccccagcaa accaccagcc cagctgtcgc catctgttcc
      901 caagagacct aaattgccag ctccaactcg taccccagcc acagccccag tccctgcccg
      961 agcacagggg gcagtgacag gcaaaccccg aggagaaggc accgagccca gacgaccccg
     1021 agctggccca gaggagctgg ggaagatcct tcagggtgtg gtagtggtgc tgagtggctt
     1081 ccagaacccc ttccgctccg agctgcgaga taaggcccta gagcttgggg ccaagtatcg
     1141 gccagactgg acccgggaca gcacgcacct catctgtgcc tttgccaaca cccccaagta
     1201 cagccaggtc ctaggcctgg gaggccgcat cgtgcgtaag gagtgggtgc tggactgtca
     1261 ccgcatgcgt cggcggctgc cctcccggag gtacctcatg gcagggccag gttccagcag
     1321 tgaggaggat gaggcctctc acagcggtgg cagcggagat gaagccccca agcttcctca
     1381 gaagcaaccc cagaccaaaa ccaagcccac tcaggcagct ggacccagct caccccagaa
     1441 gcccccaacc cctgaagaga ccaaagcagc ctcaccagtg ctccaggaag atatagacat
     1501 tgagggggta cagtcagaag gacaggacaa tggggcggaa gattctgggg acacagagga
     1561 tgagctgagg agggtggcag agcagaagga acacagactg ccccctggcc aggaggagaa
     1621 tggggaagac ccgtatgcag gctccacgga tgagaacacg gacagtgagg aacaccagga
     1681 gcctcctgat ctgccagtcc ctgagctccc agatttcttc cagggcaagc acttctttct
     1741 ttacggggag ttccctgggg acgagcggcg gaaactcatc cgatacgtca cagccttcaa
     1801 tggggagctc gaggactata tgagtgaccg ggttcagttt gtgatcacag cacaggaatg
     1861 ggatcccagc tttgaggagg ccctgatgga caacccctcc ctggcattcg ttcgtccccg
     1921 atggatctac agttgcaatg agaagcagaa gttacttcct caccagctct atggggtggt
     1981 gccgcaagcc gaattcctcg agatgtgcat ctgcggagat gcactagttg ccctacccga
     2041 gggcgagtcg gtacgcatcg ccgacatcgt gccgggtgcg cggcccaaca gtgacaacgc
     2101 catcgacctg aaagtccttg accggcatgg caatcccgtg ctcgccgacc ggctgttcca
     2161 ctccggcgag catccggtgt acacggtgcg tacggtcgaa ggtctgcgtg tgacgggcac
     2221 cgcgaaccac ccgttgttgt gtttggtcga cgtcgccggg gtgccgaccc tgctgtggaa
     2281 gctgatcgac gaaatcaagc cgggcgatta cgcggtgatt caacgcagcg cattcagcgt
     2341 cgactgtgca ggttttgccc gcgggaaacc cgaatttgcg cccacaacct acacagtcgg
     2401 cgtccctgga ctggtgcgtt tcttggaagc acaccaccga gacccggacg cccaagctat
     2461 cgccgacgag ctgaccgacg ggcggttcta ctacgcgaaa gtcgccagtg tcaccgacgc
     2521 cggcgtgcag ccggtgtata gccttcgtgt cgacacggca gaccacgcgt ttatcacgaa
     2581 cgggttcgtc agccacgcta ctggcctcac cggtctgaac tcaggcctcg gatccggaat
     2641 gtcccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt
     2701 ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg
     2761 gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg
     2821 tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat
     2881 gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga
     2941 tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga
     3001 ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac
     3061 atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt
     3121 tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa
     3181 acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg
     3241 gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatagatccg
     3301 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     3361 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     3421 atatccggat aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3481 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3541 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3601 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3661 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3721 gcggtaagat ccttgagagt tttcgccccg aagaacgttc tccaatgatg agcactttta
     3781 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     3841 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3901 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3961 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4021 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4081 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4141 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4201 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4261 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4321 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4381 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4441 aagtttactc atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa
     4501 aaacaggaag attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt
     4561 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     4621 cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa
     4681 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     4741 cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa
     4801 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     4861 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     4921 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     4981 cgcgtaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5041 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5101 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5161 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5221 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5281 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5341 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5401 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5461 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5521 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5581 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5641 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5701 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5761 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5821 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca
     5881 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     5941 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6001 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6061 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     6121 gcagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     6181 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     6241 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     6301 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
     6361 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
     6421 aatgccagcc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
     6481 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     6541 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     6601 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     6661 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     6721 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
     6781 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6841 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
     6901 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
     6961 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
     7021 gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag
     7081 atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
     7141 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt
     7201 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga
     7261 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat
     7321 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
     7381 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
     7441 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
     7501 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
     7561 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
     7621 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
     7681 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
     7741 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
     7801 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
     7861 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
     7921 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
     7981 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     8041 cgccgcaagg aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca
     8101 attccccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     8161 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     8221 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa
     8281 ttaattcccc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     8341 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     8401 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     8461 gaattaattc cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     8521 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     8581 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     8641 caggaattaa ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     8701 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
     8761 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
     8821 tgccaggaat taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     8881 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     8941 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     9001 aactgccagg aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg
     9061 cgaaat
//
