LOCUS       sequence-80855-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express T7 and GFP-tagged hAIM2 in mammalian cells  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pMSCVpuro-T7-hAIM2-EGFP
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    52..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             91..113
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             121..153
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 atctctcgag gttgatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg
       61 agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat
      121 atggctagca tgactggtgg acagcaaatg ggtcgcggat ccatggagag taaatacaag
      181 gagatactct tgctaacagg cctggataac atcactgatg aggaactgga taggtttaag
      241 ttctttcttt cagacgagtt taatattgcc acaggcaaac tacatactgc aaacagaata
      301 caagtagcta ccttgatgat tcaaaatgct ggggcggtgt ctgcagtgat gaagaccatt
      361 cgtatttttc agaagttgaa ttatatgctt ttggcaaaac gtcttcagga ggagaaggag
      421 aaagttgata agcaatacaa atcggtaaca aaaccaaagc cactaagtca agctgaaatg
      481 agtcctgctg catctgcagc catcagaaat gatgtcgcaa agcaacgtgc tgcaccaaaa
      541 gtctctcctc atgttaagcc tgaacagaaa cagatggtgg cccagcagga atctatcaga
      601 gaagggtttc agaagcgctg tttgccagtt atggtactga aagcaaagaa gcccttcacg
      661 tttgagaccc aagaaggcaa gcaggagatg tttcatgcta cagtggctac agaaaaggaa
      721 ttcttctttg taaaagtttt taatacactg ctgaaagata aattcattcc aaagagaata
      781 attataatag caagatatta tcggcacagt ggtttcttag aggtaaatag cgcctcacgt
      841 gtgttagatg ctgaatctga ccaaaaggtt aatgtcccgc tgaacattat cagaaaagct
      901 ggtgaaaccc cgaagatcaa cacgcttcaa actcagcccc ttgg
//
