LOCUS       sequence-80859-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GST-tagged hAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-hAIM2-GST
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             33..65
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatggctag catgactggt ggacagcaaa
       61 tgggtcgcgg atccatggag agtaaataca aggagatact cttgctaaca ggcctggata
      121 acatcactga tgaggaactg gataggttta agttctttct ttcagacgag tttaatattg
      181 ccacaggcaa actacatact gcaaacagaa tacaagtagc taccttgatg attcaaaatg
      241 ctggggcggt gtctgcagtg atgaagacca ttcgtatttt tcagaagttg aattatatgc
      301 ttttggcaaa acgtcttcag gaggagaagg agaaagttga taagcaatac aaatcggtaa
      361 caaaaccaaa gccactaagt caagctgaaa tgagtcctgc tgcatctgca gccatcagaa
      421 atgatgtcgc aaagcaacgt gctgcaccaa aagtctctcc tcatgttaag cctgaacaga
      481 aacagatggt ggcccagcag gaatctatca gagaagggtt tcagaagcgc tgtttgccag
      541 ttatggtact gaaagcaaag aagcccttca cgtttgagac ccaagaaggc aagcaggaga
      601 tgtttcatgc tacagtggct acagaaaagg aattcttctt tgtaaaagtt tttaatacac
      661 tgctgaaaga taaattcatt ccaaagagaa taattataat agcaagatat tatcggcaca
      721 gtggtttctt agaggtaaat agcgcctcac gtgtgttaga tgctgaatct gaccaaaagg
      781 ttaatgtccc gctgaacatt atcagaaaag ctggtgaaac cccgaagatc aacacgcttc
      841 aaactcagcc ccttggaaca attgtgaatg gtttgtttgt agtccagaa
//
