LOCUS       sequence-80861-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GST-tagged mAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-mAIM2-GST
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatggcta gcatgactgg tggacagcaa
       61 atgggtcgcg gatccatgga gagtgagtac cgggaaatgc tgttgttgac cggcctggac
      121 cacatcacgg aggaagaact gaaacggttc aagtactttg ctttgactga gtttcagatt
      181 gccaggagca cactcgacgt ggcagatagg acagagttag ctgaccacct gattcaaagt
      241 gcaggtgcgg cgtctgcagt gaccaaggcc attaatattt tccagaagtt gaattatatg
      301 catattgcaa atgctcttga agagaaaaag aaagaagctg aacgtaaact catgaccaat
      361 acaaagaaga gaggaacaca gaaggtagaa aatagaagtc aagctgaaaa ctgctctgct
      421 gcctctgcca cccgcagtga caatgacttt aaggaacagg ctgctacaga agtctgtcct
      481 caagctaagc ctcagaagaa acagatggtg gcagaacagg aagccatcag agaagattta
      541 cagaaagatc cacttgttgt cacggtgctg aaagctataa atccctttga gtgtgagact
      601 caggaaggaa gacaagagat atttcatgca acagtggcca cggagacaga ttttttcttt
      661 gtaaaagttt taaacgcaca gtttaaagat aaatttatcc caaagaggac aattaaaata
      721 tcaaactacc tttggcacag taacttcatg gaggtcacca gttcctcagt tgtggttgat
      781 gttgaatcta accacgaagt cccaaataac gttgttaaga gagccaggga aactcccagg
      841 attagtaaac tgaagattca gccatgtgga acaattgtga atgggctgtt taaagtccag
      901 aagataacag aggaaaaaga tagagtactg tatggtatac atgataaa
//
