LOCUS       sequence-80862-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express His6-tagged HIN domain of mAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-mAIM2-deltaPYD
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             847..864
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatggcta gcatgactgg tggacagcaa
       61 atgggtcgcg gatccatgac acagaaggta gaaaatagaa gtcaagctga aaactgctct
      121 gctgcctctg ccacccgcag tgacaatgac tttaaggaac aggctgctac agaagtctgt
      181 cctcaagcta agcctcagaa gaaacagatg gtggcagaac aggaagccat cagagaagat
      241 ttacagaaag atccacttgt tgtcacggtg ctgaaagcta taaatccctt tgagtgtgag
      301 actcaggaag gaagacaaga gatatttcat gcaacagtgg ccacggagac agattttttc
      361 tttgtaaaag ttttaaacgc acagtttaaa gataaattta tcccaaagag gacaattaaa
      421 atatcaaact acctttggca cagtaacttc atggaggtca ccagttcctc agttgtggtt
      481 gatgttgaat ctaaccacga agtcccaaat aacgttgtta agagagccag ggaaactccc
      541 aggattagta aactgaagat tcagccatgt ggaacaattg tgaatgggct gtttaaagtc
      601 cagaagataa cagaggaaaa agatagagta ctgtatggta tacatgataa aacagggaca
      661 atggaggtgt tggtgctggg aaacccaagc aaaacaaagt gcgaggaagg agacaagatt
      721 agactcacgt tctttgaggt gtcaaaaaat ggagtgaaaa ttcagttgaa atctggacct
      781 tgtagctttt ttaaggttat taaggctgca aagccaaaaa ctgacatgaa aagtgtggag
      841 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      901 gagttggctg ctgccaccgc tga
//
