LOCUS       sequence-80863-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GST-tagged HIN domain of mAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-mAIM2-deltaPYD-GST
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             33..65
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatggctag catgactggt ggacagcaaa
       61 tgggtcgcgg atccatgaca cagaaggtag aaaatagaag tcaagctgaa aactgctctg
      121 ctgcctctgc cacccgcagt gacaatgact ttaaggaaca ggctgctaca gaagtctgtc
      181 ctcaagctaa gcctcagaag aaacagatgg tggcagaaca ggaagccatc agagaagatt
      241 tacagaaaga tccacttgtt gtcacggtgc tgaaagctat aaatcccttt gagtgtgaga
      301 ctcaggaagg aagacaagag atatttcatg caacagtggc cacggagaca gattttttct
      361 ttgtaaaagt tttaaacgca cagtttaaag ataaatttat cccaaagagg acaattaaaa
      421 tatcaaacta cctttggcac agtaacttca tggaggtcac cagttcctca gttgtggttg
      481 atgttgaatc taaccacgaa gtcccaaata acgttgttaa gagagccagg gaaactccca
      541 ggattagtaa actgaagatt cagccatgtg gaacaattgt gaatgggctg tttaaagtcc
      601 agaagataac agaggaaaaa gatagagtac tgtatggtat acatgataaa acagggacaa
      661 tggaggtgtt ggtgctggga aacccaagca aaacaaagtg cgaggaagga gacaagatta
      721 gactcacgtt ctttgaggtg tcaaaaaatg gagtgaaaat tcagttgaaa tctggacctt
      781 gtagcttttt taaggttatt aaggctgcaa agccaaaaac tgacatgaaa agtgtggagc
      841 tcgagatgtc ccctatacta ggttattgga aaattaaggg ccttgtgcaa cccactcgac
      901 ttcttttgga atatcttgaa gaaaa
//
