LOCUS       sequence-80864-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GFP-tagged PYD of mAIM2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pMSCVpuro-mAIM2-PYD-EGFP
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(303..305,306..818)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(345..366)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(606..625)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggagag tgagtaccgg gaaatgctgt tgttgaccgg cctggaccac atcacggagg
       61 aagaactgaa acggttcaag tactttgctt tgactgagtt tcagattgcc aggagcacac
      121 tcgacgtggc agataggaca gagttagctg accacctgat tcaaagtgca ggtgcggcgt
      181 ctgcagtgac caaggccatt aatattttcc agaagttgaa ttatatgcat attgcaaatg
      241 ctcttgaaga gaaaaagaaa gaagctgaac gtaaactcat gaccaataca aagaagagag
      301 gagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      361 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      421 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      481 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      541 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      601 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      661 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      721 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      781 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      841 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      901 acctgagcac ccagtccgcc ctgagcaaag accc
//
