LOCUS       sequence-80866-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express His6-tagged hAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-hAIM2-His6
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..69
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg ctagcatgac tggtggacag
       61 caaatgggtc gcggatccat ggagagtaaa tacaaggaga tactcttgct aacaggcctg
      121 gataacatca ctgatgagga actggatagg tttaagttct ttctttcaga cgagtttaat
      181 attgccacag gcaaactaca tactgcaaac agaatacaag tagctacctt gatgattcaa
      241 aatgctgggg cggtgtctgc agtgatgaag accattcgta tttttcagaa gttgaattat
      301 atgcttttgg caaaacgtct tcaggaggag aaggagaaag ttgataagca atacaaatcg
      361 gtaacaaaac caaagccact aagtcaagct gaaatgagtc ctgctgcatc tgcagccatc
      421 agaaatgatg tcgcaaagca acgtgctgca ccaaaagtct ctcctcatgt taagcctgaa
      481 cagaaacaga tggtggccca gcaggaatct atcagagaag ggtttcagaa gcgctgtttg
      541 ccagttatgg tactgaaagc aaagaagccc ttcacgtttg agacccaaga aggcaagcag
      601 gagatgtttc atgctacagt ggctacagaa aaggaattct tctttgtaaa agtttttaat
      661 acactgctga aagataaatt cattccaaag agaataatta taatagcaag atattatcgg
      721 cacagtggtt tcttagaggt aaatagcgcc tcacgtgtgt tagatgctga atctgaccaa
      781 aaggttaatg tcccgctgaa cattatcaga aaagctggtg aaaccccgaa gatcaacacg
      841 cttcaaactc agccccttgg aacaattgtg aatggtttgt ttgtagtcca gaa
//
