LOCUS       sequence-80872-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GST-tagged PYD of hAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-hAIM2-PYD-GST
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..69
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg ctagcatgac tggtggacag
       61 caaatgggtc gcggatccat ggagagtaaa tacaaggaga tactcttgct aacaggcctg
      121 gataacatca ctgatgagga actggatagg tttaagttct ttctttcaga cgagtttaat
      181 attgccacag gcaaactaca tactgcaaac agaatacaag tagctacctt gatgattcaa
      241 aatgctgggg cggtgtctgc agtgatgaag accattcgta tttttcagaa gttgaattat
      301 atgcttttgg caaaacgtct tcaggaggag aaggagaaag ttgataagca atacaaatcg
      361 gtaacaaaac caaagccact cgagatgtcc cctatactag gttattggaa aattaagggc
      421 cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg
      481 tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt
      541 cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata
      601 cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt
      661 tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt
      721 aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg
      781 ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac
      841 ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg
      901 ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac
      961 ttgaaatcca gcaagtatat agcatg
//
