LOCUS       sequence-80873-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express T7-tagged HIN domain of hAIM2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pcDNA3-hAIM2deltPYD-T7
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(797..816)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(798..816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(861..960)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(890..910)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tttgctgtcc accagtcatg ctagccatct cgagtgtttt ttttttggcc ttaataacct
       61 ttatggtgct atgaactcca gatgtcagct gtagtttttc tccatttttt gacagtgtga
      121 agaatgtaag tcgaacctta tctccttcct tacatttcat tgtgtcctcg tttctaaccc
      181 ccagtacttc cattttccca gtgttgtcac ttaggtcaaa taatatgttt ttcttctttt
      241 ctgttacctt ctggactaca aacaaaccat tcacaattgt tccaaggggc tgagtttgaa
      301 gcgtgttgat cttcggggtt tcaccagctt ttctgataat gttcagcggg acattaacct
      361 tttggtcaga ttcagcatct aacacacgtg aggcgctatt tacctctaag aaaccactgt
      421 gccgataata tcttgctatt ataattattc tctttggaat gaatttatct ttcagcagtg
      481 tattaaaaac ttttacaaag aagaattcct tttctgtagc cactgtagca tgaaacatct
      541 cctgcttgcc ttcttgggtc tcaaacgtga agggcttctt tgctttcagt accataactg
      601 gcaaacagcg cttctgaaac ccttctctga tagattcctg ctgggccacc atctgtttct
      661 gttcaggctt aacatgagga gagacttttg gtgcagcacg ttgctttgcg acatcatttc
      721 tgatggctgc agatgcagca ggactcattt cagcttgact tagcatggat ccgagctcgg
      781 taccaagctt gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg
      841 ggttctctag ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg
      901 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
      961 tgccaaaaca aactcccatt gacgtcaatg gg
//
