LOCUS       sequence-80874-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express His6-tagged HIN domain of hAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-hAIM2-deltaPYD
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..77
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             825..842
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(895..913)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      909..956
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatggct agcatgactg
       61 gtggacagca aatgggtcgc ggatccatgc taagtcaagc tgaaatgagt cctgctgcat
      121 ctgcagccat cagaaatgat gtcgcaaagc aacgtgctgc accaaaagtc tctcctcatg
      181 ttaagcctga acagaaacag atggtggccc agcaggaatc tatcagagaa gggtttcaga
      241 agcgctgttt gccagttatg gtactgaaag caaagaagcc cttcacgttt gagacccaag
      301 aaggcaagca ggagatgttt catgctacag tggctacaga aaaggaattc ttctttgtaa
      361 aagtttttaa tacactgctg aaagataaat tcattccaaa gagaataatt ataatagcaa
      421 gatattatcg gcacagtggt ttcttagagg taaatagcgc ctcacgtgtg ttagatgctg
      481 aatctgacca aaaggttaat gtcccgctga acattatcag aaaagctggt gaaaccccga
      541 agatcaacac gcttcaaact cagccccttg gaacaattgt gaatggtttg tttgtagtcc
      601 agaaggtaac agaaaagaag aaaaacatat tatttgacct aagtgacaac actgggaaaa
      661 tggaagtact gggggttaga aacgaggaca caatgaaatg taaggaagga gataaggttc
      721 gacttacatt cttcacactg tcaaaaaatg gagaaaaact acagctgaca tctggagttc
      781 atagcaccat aaaggttatt aaggccaaaa aaaaaacact cgagcaccac caccaccacc
      841 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      901 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      961 aaggaggaac tatatcc
//
