LOCUS       sequence-80878-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T
  TITLE     Reversible dimerization of Aequorea victoria fluorescent proteins 
            increases the dynamic range of FRET-based indicators.
  JOURNAL   ACS Chem Biol. 2010 Feb 19;5(2):215-22. doi: 10.1021/cb900263z.
  PUBMED    20047338
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2010-02-19- 19"; volume: "5"; issue: "2"; 
            pages: "215-22"
COMMENT     Sequence Label: Ateam1.03-nD/nA/pENTR1A
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(103..105,106..108,109..621)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(148..169)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(409..428)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     756..777
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggcttttcga gtatggtgag caagggcgag
      121 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      181 aggttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      241 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      301 tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      361 tccgccatgc ccgaaggcta cgtccaggag cgtaccatct tcttcaagga cgacggcaac
      421 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      481 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      541 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggcccacttc
      601 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
      661 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      721 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      781 gccgccatcg atatgaaaac tgtgaaagtg aatataacaa cccctgatgg gccagtctac
      841 gacgctgata tcgagatggt gtccgtgcgg gccgagagtg gtgatctcgg catcc
//
