LOCUS       sequence-80880-e        2151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human SPOP (aa 28-337) fused to His-MBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, 
            Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, 
            White KP, Schulman BA
  TITLE     Structures of SPOP-substrate complexes: insights into molecular 
            architectures of BTB-Cul3 ubiquitin ligases.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
  PUBMED    19818708
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "39-50"
COMMENT     Sequence Label: His-MBP human SPOP (aa 28-337)
FEATURES             Location/Qualifiers
     source          1..2151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..1140
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1114..1137
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1195..1215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggttctt ctcaccatca ccatcaccac ggttcttcta tgaaaatcga agaaggtaaa
       61 ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa
      121 ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa
      181 ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc
      241 tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag
      301 gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac
      361 ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca
      421 aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg
      481 ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt
      541 tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct
      601 ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca
      661 gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc
      721 aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta
      781 ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt
      841 aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact
      901 gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct
      961 tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa
     1021 ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg
     1081 gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact
     1141 aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgaggaaaac
     1201 ctgtattttc agggatccaa agtagtgaaa ttctcctaca tgtggaccat caataacttt
     1261 agcttttgcc gggaggaaat gggtgaagtc attaaaagtt ctacattttc atcaggagca
     1321 aatgataaac tgaaatggtg tttgcgagta aaccccaaag ggttagatga agaaagcaaa
     1381 gactacctgt cactttacct gttactggtc agctgtccaa agagtgaagt tcgggcaaaa
     1441 ttcaaattct ccatcctgaa tgccaaggga gaagaaacca aagctatgga gagtcaacgg
     1501 gcatataggt ttgtgcaagg caaagactgg ggattcaaga aattcatccg tagagatttt
     1561 cttttggatg aggccaacgg gcttctccct gatgacaagc ttaccctctt ctgcgaggtg
     1621 agtgttgtgc aagattctgt caacatttct ggccagaata ccatgaacat ggtaaaggtt
     1681 cctgagtgcc ggctggcaga tgagttagga ggactgtggg agaattcccg gttcacagac
     1741 tgctgcttgt gtgttgccgg ccaggaattc caggctcaca aggctatctt agcagctcgt
     1801 tctccggttt ttagtgccat gtttgaacat gaaatggagg agagcaaaaa gaatcgagtt
     1861 gaaatcaatg atgtggagcc tgaagttttt aaggaaatga tgtgcttcat ttacacgggg
     1921 aaggctccaa acctcgacaa aatggctgat gatttgctgg cagctgctga caagtatgcc
     1981 ctggagcgct taaaggtcat gtgtgaggat gccctctgca gtaacctgtc cgtggagaac
     2041 gctgcagaaa ttctcatcct ggccgacctc cacagtgcag atcagttgaa aactcaggca
     2101 gtggatttta tcaactatca tgcttcggat gtcttggaga cctctgggta a
//
