LOCUS       sequence-80883-e        5385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter for normal human GPR56 exon 1m promoter activity. Human 
            GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted
            into pGL3E..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang 
            PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, 
            Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh 
            CA
  TITLE     Evolutionarily dynamic alternative splicing of GPR56 regulates 
            regional cerebral cortical patterning.
  JOURNAL   Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392.
  PUBMED    24531968
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-02-14- 14"; volume: "343"; 
            issue: "6172"; pages: "764-8"
COMMENT     Sequence Label: pGL3E-hGPR56 e1m promoter normal
FEATURES             Location/Qualifiers
     source          1..5385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..2061
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(475..493)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2102..2223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2113..2132)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2167..2186
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2545..2565
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(2717..2734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2888..3476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2968..2987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3647..4438,4439..4507))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4270..4289
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4508..4612)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4639..5094
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4726..4745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4936..4957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5225..5273
                     /note="synthetic polyadenylation signal"
     misc_feature    5287..5378
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5327..5346
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttcc
       61 ccataaatcg ctgtcctaac ccctgccctc cctcctgcca gctccctgtc tggcctgggc
      121 agcgtctgag ttgaggactt gggaacagga caagttacgg agccacgttg ctttgctggg
      181 tctgagccgg ggtgtgacgt agtccctgca gctgccaacg gttgccaggg caacggttgc
      241 caggggctgc tgtcacctgc gccccttctc ccgcgctggc ggctggggct tctcagcctc
      301 tattccctgg ctgtcccctt tgtttgaagc tccagtgagg gagcagtggc tggggtggcc
      361 cagcttcaaa gtcaagcttg gcattccggt actgttggta aagccaccat ggaagacgcc
      421 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
      481 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
      541 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
      601 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
      661 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
      721 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
      781 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
      841 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
      901 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
      961 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     1021 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     1081 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     1141 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     1201 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     1261 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     1321 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     1381 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     1441 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     1501 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     1561 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     1621 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     1681 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     1741 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     1801 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     1861 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     1921 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     1981 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     2041 ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg
     2101 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     2161 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     2221 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     2281 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct gaacgatgga
     2341 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     2401 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     2461 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     2521 ctggggagcc tggggacttt ccacacccta actgacacac attccacagc ggatccgtcg
     2581 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     2641 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     2701 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2761 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2821 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2881 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2941 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3001 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3061 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3121 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3181 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3241 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3301 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3361 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3421 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3481 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3541 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3601 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3661 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3721 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3781 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3841 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3901 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3961 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4021 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4081 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4141 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4201 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4261 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4321 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4381 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4441 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4501 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4561 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4621 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4681 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4741 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4801 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4861 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4921 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4981 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5041 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgccatt
     5101 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5161 gccagcccaa gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg
     5221 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     5281 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     5341 tgtccccagt gcaagtgcag gtgccagaac atttctctat cgata
//
