LOCUS       sequence-80885-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-activated AAV expression of humanized ChR2 with H134R mutation 
            fused to EYFP for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  TITLE     Cre-activated AAV expression plasmids
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE...
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(34..960)
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      957..966
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcggccgc tggcacggct ccggcctcgg cttcgtcttc
       61 gacgagagtc tcgacctcga tctccgttcc gccgatgttc agtttggtgg ttttgcggat
      121 atctccgtga atcaatatgt gctcgtggat caggacgcgc aggtagtgtc ccaacaaccc
      181 ccaacaattt ttactcatca gatcaataat cgtgtgacct acggtggagc catagacgct
      241 caggacgcca aaaccttcgg gccccaaaat gaagagaatt gggaacatac cccagctcac
      301 gaaaaacagc catgccatgc cggtcacgac ctggcggcac cgaccctttg gcacagtatg
      361 ataaccctcg atatatgctt tggcggcgtg aaaaaatgtg ttcgcgccat agcacaatcc
      421 aagacaaaag aagatgactt taacatagcc ggttgccatg gcgctggtag ccccccacac
      481 gatagtcccg atgtctgaga caaggagtcc catggttctc ctgctgtagt cgttgctcag
      541 gccggtgagg ttgctcaggc ggataaggat gacaggacaa gtgagcagcc actctgcata
      601 gcgcagccac tgcacccggt gtcctgtggc aaggtagagc atagagggat tcttaaactc
      661 aaaaaagaac tcgagaatca ccttaaccat ttcaatggcg cacacataga tctcctccca
      721 gccgcatgta gatttccagg tttggtaggc atagaacatc agcagcaaaa tgctgaatcc
      781 tgctgcaagc cactgcagga catttgacgc ggtctgagcg ccgttcgtgc cgcgagattc
      841 aatccatccg gcacagtaac attgatcctc agggaccagg acggacccgt tcaccaccac
      901 aggattagta acgaacaaaa gttcgcgtcc gacggcagac aaagcgccgc catagtccat
      961 ggtggctagc ataacttcgt
//
