LOCUS       sequence-80886-e        3457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and 
            Rbx1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, 
            Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, 
            White KP, Schulman BA
  TITLE     Structures of SPOP-substrate complexes: insights into molecular 
            architectures of BTB-Cul3 ubiquitin ligases.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
  PUBMED    19818708
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "39-50"
COMMENT     Sequence Label: Split 'n coexpress CUL3-RBX1
FEATURES             Location/Qualifiers
     source          1..3457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     612..634
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             661..681
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     RBS             3110..3132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
       61 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      121 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      181 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      241 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      301 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      361 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      421 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      481 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      541 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      601 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      661 gaaaacctgt attttcaggg atccccgaat tcccgggcta tgtcgaatct tagcaaaggc
      721 acgggcagcc ggaaggacac caagatgcgg atccgggcct ttccgatgac catggatgaa
      781 aaatatgtaa acagcatttg ggaccttctg aaaaatgcaa ttcaagaaat ccagcgtaag
      841 aataacagtg gtcttagttt tgaggagctc tatagaaatg catatacaat ggttttgcat
      901 aaacatggag aaaagctcta cactggacta agagaagttg ttaccgaaca tctcataaat
      961 aaggtgcgag aagatgtact aaattcattg aataacaact ttcttcaaac gctaaatcaa
     1021 gcttggaatg atcatcaaac agctatggtg atgattagag acatactaat gtacatggac
     1081 cgtgtgtatg tacaacaaaa taatgtggag aacgtctaca atttgggatt aattattttt
     1141 cgagatcaag ttgtacgtta tgggtgtatt agggatcatc tacggcaaac tctattggat
     1201 atgattgcaa gagagcggaa aggagaagtc gtagacagag gcgcaataag aaatgcttgc
     1261 cagatgttaa tgattttagg tctcgaagga agatcagtct atgaagaaga ttttgaggct
     1321 ccttttttgg aaatgtctgc agaatttttt cagatggaaa gccagaaatt tttagcagaa
     1381 aatagtgctt cagtatatat aaagaaagta gaagctagaa ttaatgaaga aatagaacga
     1441 gtgatgcact gccttgacaa atcaacggaa gaaccaattg taaaggtggt tgaaagggaa
     1501 ctcatttcca agcacatgaa gactatagta gaaatggaga attctgggct agtacatatg
     1561 ttgaaaaatg gaaagacaga agaccttggt tgcatgtaca agttatttag tcgtgtgcca
     1621 aatggttaac tcgagtagaa ataattttgt ttaacttaag aaggagatat acatatgaag
     1681 ctttctagaa gatctggtac cttgaaaaca atgtgtgagt gtatgagttc ctatttgagg
     1741 gagcaaggta aagctcttgt ttctgaagaa ggagaaggaa agaatcctgt tgactatatc
     1801 cagggcttat tggatctgaa gagtaggttc gatcgcttcc tcctggaatc attcaacaat
     1861 gaccgtctct ttaaacaaac tattgcgggt gactttgagt attttctcaa cctcaactcc
     1921 aggtctcctg aatacctctc attatttatt gatgataagc tgaaaaaggg agtcaaaggg
     1981 ctaacagaac aagaagtaga aacaatattg gataaagcaa tggtcctttt taggtttatg
     2041 caagaaaaag atgtatttga acgttattat aaacaacact tggcaaggag acttctcaca
     2101 aataaaagtg tttctgatga ctctgaaaaa aacatgatat ctaagttaaa gactgaatgt
     2161 ggatgtcagt tcacgtcaaa actggaagga atgtttaggg atatgagcat ctcaaacaca
     2221 acgatggatg aattcaggca acatctacag gcaactggtg tatctttagg tggtgttgat
     2281 cttacagtcc gggtgctcac gacaggatat tggcccactc agtcagccac accaaagtgc
     2341 aacatcccac cagcaccaag acatgctttt gagatattca gaaggttcta cttagccaaa
     2401 cacagtggtc gacagctcac actccagcat catatgggtt ctgcagatct caatgccaca
     2461 ttttatggac cagttaaaaa ggaagatgga tctgaagttg gtgttggagg tgcacaagta
     2521 actggctcta atacacggaa gcacatattg caagtttcca ctttccagat gaccatatta
     2581 atgctcttta ataatagaga aaaatacaca tttgaggaaa ttcagcaaga gacagatatc
     2641 cctgaaagag agcttgttag agccctacag tccctcgcct gtggtaaacc aacacagcgg
     2701 gttcttacaa aagaacccaa atcaaaggaa atagaaaatg gtcatatatt tacagttaat
     2761 gatcaattca catccaaact acacagagtc aagattcaaa cagttgctgc caaacaaggt
     2821 gaatccgacc cagagaggaa agaaacaagg cagaaagtag acgacgacag aaaacatgag
     2881 atagaagctg ctatagtgcg gataatgaaa tctagaaaga agatgcagca caatgttcta
     2941 gtagcggagg taactcagca gttgaaggcg cgattcttac caagtccagt tgttattaag
     3001 aaacgtattg aaggacttat tgagagagaa tatttggcac gaacacctga ggatcgcaaa
     3061 gtatacacat atgtagcata aggtaccgcc atgcggccgc agaaataatt ttgtttaact
     3121 ttaagaagga gatataatcg atatggatgt ggataccccc agcggcacca acagcggcgc
     3181 gggcaagaag cgctttgaag ttaaaaagtg gaatgcagtg gccctctggg cctgggacat
     3241 tgtggttgat aactgtgcca tctgcaggaa ccacattatg gatctttgta tcgaatgtca
     3301 ggccaaccag gcgtcagcta cttccgaaga gtgtacggtt gcatggggag tctgcaacca
     3361 tgcttttcat ttccactgca tctctcgatg gctcaaaacg aggcaggtgt gtccgttgga
     3421 caacagagag tgggagttcc agaagtatgg gcattag
//
