LOCUS       sequence-80889-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Phamret, a monomeric photoconvertible 
            fluorescent protein, fused to H2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Matsuda T, Miyawaki A, Nagai T
  TITLE     Direct measurement of protein dynamics inside cells using a 
            rationally designed photoconvertible protein.
  JOURNAL   Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 
            2008 Mar 16.
  PUBMED    18345008
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1193"; journalName: "Nat Methods"; date: "2008-04"; 
            volume: "5"; issue: "4"; pages: "339-45"
COMMENT     Sequence Label: Phamret-H2B/pcDNA3
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(457..478)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     806..825
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atagggccct ctagatgcat gctcgagtta cttagcgctg gtgtacttgg tgacggcctt
       61 ggtgccctcg gacacggcgt gcttggccaa ctccccgggc agcagcaggc gcacggccgt
      121 ctggatctcc ctggaggtga tggtcgagcg cttgttgtaa tgcgccaggc gggaagcctc
      181 acccgcgatg cgttcgaaga tgtcgttgac gaaggagttc atgattccca tggccttaga
      241 ggagatgccg gtgtcggggt ggacctgctt cagcaccttg tacacgtata cggagtagct
      301 ctccttgcgg ctgcgcttgc gcttcttgcc gtccttcttc tgcgccttag tcacggcttt
      361 cttcgagccc ttcttgggcg cgggagcaga cttcgctggc tccggcatga attccttgta
      421 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      481 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tggctcaggt agtggttgtc
      541 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      601 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      661 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
      721 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      781 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      841 gcgctcctgg acgtagcctt cgggcatggc gga
//
