LOCUS       sequence-80893-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for C-term eGFP fusion, frame N2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR1A-GFP-N2 (FR1)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    161..260
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(330..351)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     679..698
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatcgcggcc tcgacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
       61 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
      121 cagagatttt gagacacggg ccagagctgc agctggatgg caaataatga ttttattttg
      181 actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttctta taatgccaac
      241 tttgtacaag aaagctgggt ctagatatct cgagtgcggc cgctttactt gtacagctcg
      301 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      361 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
      421 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
      481 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
      541 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
      601 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      661 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      721 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
      781 tagcggctga agcactgcac
//
