LOCUS       sequence-80894-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of SEGFP, a super enhanced green fluorescent 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     Nagai lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SEGFP/pcDNA3
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(90..92,93..95,96..809)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(135..156)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(396..415)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     743..764
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttgcg gccgccacca tggtgagcaa gggcgaggag ctgttcaccg
      121 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      181 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      241 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      301 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      361 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      421 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      481 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      541 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
      601 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      661 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      721 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      781 ctctcggcat ggacgagctg tacaagtaag aattctgcag atatccatca cactggcggc
      841 cgctcgagca tgcatctaga gggccctatt cta
//
