LOCUS       sequence-80895-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for C-term eGFP fusion, frame N2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR1A-GFP-N2 (FR1)
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    39..91
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(120..219)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(238..261)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(269..296)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(388..474)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(719..738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctctcgccc ttgctcacca tggtggcgac cggccggtgg atcccgggcc cgcggtaccg
       61 tcgactgcag aattcgaagc ttgagctcga gatccagtcg actgaattgg ttcctttaaa
      121 gcctgctttt ttgtacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa
      181 cgaacaggtc actatcagtc aaaataaaat cattatttgg ggcccgagct taagtaacta
      241 actaacagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      301 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
      361 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
      421 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
      481 ggcagttccc tactctcgct tagtagttag acgtccccga gatccatgct agcggtaata
      541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      721 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      781 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      841 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
//
