LOCUS       sequence-8090-ex        5221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L, Helenius A
  TITLE     Assembly and trafficking of caveolar domains in the cell: caveolae 
            as stable, cargo-triggered, vesicular transporters.
  JOURNAL   J Cell Biol. 2005 Aug 29. 170(5):769-79.
  PUBMED    16129785
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2005 Aug 29. 170(5):769-79."
COMMENT     Sequence Label: Cav1-mRFP
FEATURES             Location/Qualifiers
     source          1..5221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1194..1203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1200..1874
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1200..1874
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1330..1348)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1598..1617
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1792..1815
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2009..2130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2046..2065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2100..2119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2137..2592)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2274..2295)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2486..2505
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2619..2723
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2725..3082
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2725..2916
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2748..2768)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2933..3068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3117..3911
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3117..3911
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3171..3190)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3781..3800
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4099..4118)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4143..4190
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4519..5107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5008..5027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcggcttg gatgtctggg ggcaaatacg
      661 tagactccga ggggcacctc tacaccgttc ccatccggga gcagggcaac atctacaagc
      721 ccaacaacaa ggccatggcg gaggagatga gcgagaagca ggtgtacgac gcgcacacca
      781 aggaaatcga cctggtcaac cgcgacccca agcatctcaa cgacgacgtg gtcaagattg
      841 attttgaaga tgtgattgca gaaccagaag gaacacacag ttttgatggc atctggaagg
      901 ccagcttcac caccttcact gtgacaaaat actggtttta ccgcttgctg tctgccctct
      961 ttggcatccc aatggcactc atatggggca tttactttgc cattctttct ttcctgcaca
     1021 tctgggcagt tgtgccgtgc attaagagtt tcctgattga gattcagtgc atcagccgtg
     1081 tctattccat ctacgtccac accttctgtg acccgttctt tgaggctgtt ggcaaaatat
     1141 tcagcaatat ccgcatcaac atgcagaaag aaacagggat ccaccggccg gtcgccacca
     1201 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct
     1261 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1321 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     1381 tgtcccctca gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1441 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1501 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     1561 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1621 tgggctggga ggcctccacc gagcggatgt accccgagga cggcgccctg aagggcgaga
     1681 tcaagatgag gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct
     1741 acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc aagctggaca
     1801 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc
     1861 actccaccgg cgccctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     1921 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1981 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2101 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     2161 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2221 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2281 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2341 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2401 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2461 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2521 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2581 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2641 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2701 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     2761 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2821 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2881 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2941 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3001 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3061 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     3121 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3181 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3241 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     3301 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3361 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3421 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3481 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3541 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3601 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     3661 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3721 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3781 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3841 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3901 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3961 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4021 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4081 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4141 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     4201 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4261 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4321 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     4381 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4441 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4501 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4561 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4621 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4681 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4741 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4801 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4861 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4921 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4981 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5041 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5101 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5161 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     5221 t
//
