LOCUS       sequence-80910-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express T7 and GFP-tagged hAIM2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pEGFP-N1-T7-hAIM2
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     70..89
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        70..88
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    89..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             128..150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             158..190
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgatc
       61 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
      121 aaataatttt gtttaacttt aagaaggaga tatacatatg gctagcatga ctggtggaca
      181 gcaaatgggt cgcggatcca tggagagtaa atacaaggag atactcttgc taacaggcct
      241 ggataacatc actgatgagg aactggatag gtttaagttc tttctttcag acgagtttaa
      301 tattgccaca ggcaaactac atactgcaaa cagaatacaa gtagctacct tgatgattca
      361 aaatgctggg gcggtgtctg cagtgatgaa gaccattcgt atttttcaga agttgaatta
      421 tatgcttttg gcaaaacgtc ttcaggagga gaaggagaaa gttgataagc aatacaaatc
      481 ggtaacaaaa ccaaagccac taagtcaagc tgaaatgagt cctgctgcat ctgcagccat
      541 cagaaatgat gtcgcaaagc aacgtgctgc accaaaagtc tctcctcatg ttaagcctga
      601 acagaaacag atggtggccc agcaggaatc tatcagagaa gggtttcaga agcgctgttt
      661 gccagttatg gtactgaaag caaagaagcc cttcacgttt gagacccaag aaggcaagca
      721 ggagatgttt catgctacag tggctacaga aaaggaattc t
//
