LOCUS       sequence-80913-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For knock-in donor vector by CRISPR/CAS system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R
  TITLE     One-Step Generation of Mice Carrying Reporter and Conditional 
            Alleles by CRISPR/Cas-Mediated Genome Engineering.
  JOURNAL   Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 
            10.1016/j.cell.2013.08.022.
  PUBMED    23992847
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Aug 27. pii: S0092-8674(13)01016-7. doi: 
            10.1016/j.cell.2013.08.022."
COMMENT     Sequence Label: Oct4-IRES-eGFP-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(104..123)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     714..733
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
       61 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
      121 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
      181 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
      241 catcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
      301 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
      361 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
      421 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
      481 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
      541 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
      601 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
      661 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
      721 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
      781 caatggccga tcccatattg gctgca
//
