LOCUS       sequence-80915-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-21a(+)/MBP-His-PINK1
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(688..699)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctctagaata attttgttta actttaagaa ggagatatac atatgaaaat tgaagaaggt
       61 aaactggtca tctggattaa cggtgataaa ggctacaacg gtctggctga agtgggtaaa
      121 aaattcgaaa aagataccgg cattaaagtg acggttgaac atccggacaa actggaagaa
      181 aaatttccgc aggtggcagc aaccggtgat ggtccggaca ttatcttttg ggcacacgat
      241 cgtttcggcg gttatgccca gagtggcctg ctggcagaaa tcacgccgga taaagccttt
      301 caagacaaac tgtacccgtt cacctgggat gctgttcgct ataatggtaa actgattgcc
      361 tacccgatcg cagtcgaagc tctgagtctg atttataaca aagatctgct gccgaatccg
      421 ccgaaaacgt gggaagaaat cccggcgctg gacaaagaac tgaaagctaa gggtaaatcc
      481 gcgctgatgt ttaacctgca ggaaccgtat ttcacctggc cgctgattgc agctgatggc
      541 ggttatgcct tcaaatacga aaacggcaaa tacgatatca aagacgtcgg tgtggataac
      601 gcgggcgcca aagcaggtct gacgtttctg gttgacctga tcaaaaacaa acacatgaac
      661 gctgataccg actatagtat cgcggaagcg gccttcaata aaggcgaaac cgctatgacg
      721 attaacggtc cgtgggcgtg gagtaatatc gatacctcca aagtgaacta cggcgttacc
      781 gtcctgccga cgtttaaggg tcagccgtcc aaaccgttcg tgggcgttct gagcgccggt
      841 attaacgcag cttctccgaa taaagaactg gcaaaagaat ttctggaaaa ttattgc
//
