LOCUS       sequence-80948-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Deuschle K, Okumoto S, Fehr M, Looger LL, Kozhukh L, Frommer WB
  TITLE     Construction and optimization of a family of genetically encoded 
            metabolite sensors by semirational protein engineering.
  JOURNAL   Protein Sci. 2005 Sep . 14(9):2304-14.
  PUBMED    16131659
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Sep . 14(9):2304-14."
COMMENT     Sequence Label: pRSET FLII32Pglu-2.2m
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(494..515)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     843..862
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgcggtatc taactgtaac tgttcagttt tgatgccttt atcgttcaat tctttaatca
       61 cgtaagtggt acgtgcttct gcatccggat ggcccggttc acctttcagc agtacgaact
      121 gaatctgacc gtctttgttc agatcccaac cctgattcgc cgcccagtgt ttagcaatca
      181 aatcgccttg aataatgccg gactctttgg agtcagtgcc aacgtagtag gctttgtcgt
      241 agctatccag cgccttacga gacggttctt tgttgaagaa aaccaccggc acgttttgcc
      301 cacgcgcttt ctcaatcacc gtacccgcag ctgccgggtc aaccaggttg attgccagtg
      361 ccttcacccc tttcgccagc aatacgtcga tctgatcgtt ctgcttggac tggtcattct
      421 gagaatcatt catcagcagc tgaactcggc taccgtatag ctcgtccatg ccgagagtga
      481 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      541 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      601 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
      661 ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt
      721 tgtggctgat gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      781 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      841 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
      901 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
      961 gcacgcccca g
//
