LOCUS       sequence-8095-ex        5335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1701
FEATURES             Location/Qualifiers
     source          1..5335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             326..1042
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(356..384)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     979..999
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1058..1144
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1833..2495)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2496..3265
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(4548..5081)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5270..5275,5276..5292,5293..5298))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccatgggcaa atgggattgg ctttactaag ctgatccggt ggatgacctt ttgaatgacc
       61 tttaatagat tatattacta attaattggg gaccctagag gtcccctttt ttattttaaa
      121 aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac cggatctaga
      181 tatcgattaa ttaagtcgac atctagagtt taaactttaa ttaagcccgg gctgcagccc
      241 gggggatcca ctaggttaac gcatgcgggc ccggtaccag gcctagatag atagagagag
      301 agagagacta gtggaggaag aaaaaatgag taaaggagaa gaacttttca ctggagttgt
      361 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      421 gggtgaaggt gatgctacat acggaaagct tacccttaaa tttatttgca ctactggaaa
      481 actacctgtt ccatggccaa cacttgtcac tactttgacc tatggtgttc aatgcttttc
      541 ccgttatccg gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
      601 tgtacaggaa cgcactatat ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt
      661 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aaaggtattg attttaaaga
      721 agatggaaac attctcggac acaaactcga gtacaactat aactcacaca atgtatacat
      781 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacattga
      841 agatggatcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      901 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      961 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg
     1021 catggatgag ctctacaaat aatgaattcc caggcatcaa ataaaacgaa aggctcagtc
     1081 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     1141 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     1201 acgcccgcca taaactgcca gggaattcca actgagcgcc ggtcgctacc attaccaact
     1261 tgtctggtgt caaaaataat aggattttgt taaaattcgc gttaaatttt tgttaaatca
     1321 gctcattttt taaccaatag gccgactgcg atgagtggca gggcggggcg taattttttt
     1381 aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg
     1441 gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt
     1501 taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg
     1561 accgtgtgct tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata
     1621 attgacgata tgatcattta ttctgcctcc cagagcctga taaaaacggt gaatccgtta
     1681 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
     1741 acgcggggag gcagacaagg tatagggcgg cgaggcggct acagccgata gtctggaaca
     1801 gcgcacttac gggttgctgc gcaacccaag tgctaccggc gcggcagcgt gacccgtgtc
     1861 ggcggctcca acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc
     1921 tgctgcccgc gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg
     1981 cccggaatgc cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg
     2041 cccggataca gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg
     2101 tcgtcgtgat caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc
     2161 tggccccacg ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc
     2221 ttcacgacgg agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc
     2281 aaagaacgtc cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg
     2341 cccatctgcg ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc
     2401 ccggcccacg cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg
     2461 gcttgaatgc cgatttctct ggactgcgtg gccatgctta tctccatgcg gtagggtgcc
     2521 gcacggttgc ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga caacaattat
     2581 gcgttgcgta aaagtggcag tcaattacag attttcttta acctacgcaa tgagctattg
     2641 cggggggtgc cgcaatgagc tgttgcgtac cccccttttt taagttgttg atttttaagt
     2701 ctttcgcatt tcgccctata tctagttctt tggtgcccaa agaagggcac ccctgcgggg
     2761 ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc cggggcttgt
     2821 tgatcgactg cgcggccttc ggccttgccc aaggtggcgc tgcccccttg gaacccccgc
     2881 actcgccgcc gtgaggctcg gggggcaggc gggcgggctt cgccttcgac tgcccccact
     2941 cgcataggct tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg
     3001 agccttgacc cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc
     3061 cggaggcttt tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca
     3121 gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca
     3181 agctcgtggg caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga
     3241 gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa
     3301 gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg cgccgcagcc
     3361 gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga gtatactcaa gcattgagtg
     3421 gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac caaaagggag
     3481 gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa atgggcaacg
     3541 tggcggccag tctcaagcac gcctaccgcg agcgcgagac gcccaacgct gacgccagca
     3601 ggacgccaga gaacgagcac tgggcggcca gcagcaccga tgaagcgatg ggccgactgc
     3661 gcgagttgct gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag tacgtcatga
     3721 cggccagccc ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg ttcttcgaga
     3781 aggcgcacaa gtggctggcg gacaagtacg gggcggatcg catcgtgacg gccagcatcc
     3841 accgtgacga aaccagcccg cacatgaccg cgttcgtggt gccgctgacg caggacggca
     3901 ggctgtcggc caaggagttc atcggcaaca aagcgcagat gacccgcgac cagaccacgt
     3961 ttgcggccgc tgtggccgat ctagggctgc aacggggcat cgagggcagc aaggcacgtc
     4021 acacgcgcat tcaggcgttc tacgaggccc tggagcggcc accagtgggc cacgtcacca
     4081 tcagcccgca agcggtcgag ccacgcgcct atgcaccgca gggattggcc gaaaagctgg
     4141 gaatctcaaa gcgcgttgag acgccggaag ccgtggccga ccggctgaca aaagcggttc
     4201 ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg cgcaagaagg
     4261 ccgatcaagc ccaagagacg gcccgagacc ttcgggagcg cctgaagccc gttctggacg
     4321 ccctggggcc gttgaatcgg gatatgcagg ccaaggccgc cgcgatcatc aaggccgtgg
     4381 gcgaaaagct gctgacggaa cagcgggaag tccagcgcca gaaacaggcc cagcgccagc
     4441 aggaacgcgg gcgcgcacat ttccccgaaa agtgccacct ggcggcgttg tgacaattta
     4501 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     4561 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     4621 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     4681 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
     4741 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaacct gggcagaacg
     4801 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     4861 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     4921 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     4981 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     5041 cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat
     5101 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     5161 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     5221 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
     5281 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgt
//
